Evidence map›Paper›PMID 40100601›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Flow Cytometry Analysis of Graft Conditioning on the T-Cell Response in Graft-Versus-Host Disease Following Murine Allogeneic Transplantation.

L Angel Ayala, Erika S Varady, Cuiwen Zhou, Xiyu Chen, Abigail Valdovinos, Alizeh H Ahmedani, Gabriella L Hisoire, Pauline U Nguyen, Vanessa M Scarfone, Matthew A Inlay

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

L Angel AyalaSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Erika S VaradySue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Cuiwen ZhouSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Xiyu ChenSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Abigail ValdovinosSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Alizeh H AhmedaniSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Gabriella L HisoireSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Pauline U NguyenSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Vanessa M ScarfoneSue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA.
Matthew A InlaySue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA. minlay@uci.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acute graft-versus-host disease (aGVHD) remains a common life-threatening complication associated with allogeneic hematopoietic cell transplantation (allo-HCT) and is a major contributor to posttransplant mortality and morbidity. In allo-HCT, allogeneic T cells recognize host tissues as foreign and mount an immune response, causing inflammation and tissue damage. Traditionally, GVHD patients receive a regimen of immunosuppressive glucocorticoids after the onset of symptoms; however, many groups are developing prophylactic strategies due to the consequences associated with long-term glucocorticoid administration. Despite the advances made to mitigate GVHD, it remains a risk following allo-HCT; thus, new therapies are required to improve the safety of this therapy. Here, we use a prophylactic strategy that leverages graft manipulation as a way to induce tolerance upon transplantation. This chapter focuses on the techniques involved in conditioning donor cells, transplantation, and posttransplantation analysis of immune cells in a murine major histocompatibility complex (MHC) mismatched model of GVHD.

Indexed as

Flow CytometryGraft vs Host DiseaseHematopoietic Stem Cell TransplantationT-LymphocytesTransplantation ConditioningAnimalsDisease Models, AnimalMiceMice, Inbred C57BLTransplantation, HomologousAllogeneic hematopoietic cell transplantationConventional T cellGraft conditioningGraft-versus-host diseaseIntracellular flow cytometryRegulatory T cell

Identifiers

PMID40100601

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.