Evidence map›Paper›PMID 40100362›Full record

ArticleArchives of microbiology2025

A novel antifungal chitinase from Chaetomium globosum: column-free purification and characterization.

Cheng Jiang, Luli Li, Jialu Li

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Highly efficient chitinase production fromFrontiers in microbiology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Cheng JiangSchool of Biological Engineering, Huainan Normal University, Huainan, People's Republic of China. jiangcheng0613@163.com.ORCID http://orcid.org/0000-0002-5324-7184
Luli LiHuainan No.2 Senior High School, Huainan, People's Republic of China.
Jialu LiSchool of Biological Engineering, Huainan Normal University, Huainan, People's Republic of China.

Funding

Foundation of Huainan Normal University 2023HX024
6 · The paper itself

Abstract

A new chitinase gene, cloned from the biocontrol Chaetomium globosum W7, was designated Cgchi18. Recombinant protein Cgchi18 with 535 amino acids was expressed in Escherichia coli, and purified by means of a column-free purification method relying on split intein, achieving a 12.39-fold purification and a 15.61% recovery yield. The maximum activity of this approximately 60-kDa protein was observed at 45 °C and pH 5.0. Cgchi18 was activated by Mg

Indexed as

Antifungal AgentsChaetomiumChitinasesFungal ProteinsChitinCloning, MolecularEnzyme StabilityEscherichia coliHydrogen-Ion ConcentrationKineticsRecombinant ProteinsSubstrate SpecificityTemperatureAntifungal AgentsChitinChitinasesFungal ProteinsRecombinant ProteinsAntifungalChaetomium globosumChitinaseColumn-free purificationHydrolysis

Identifiers

PMID40100362

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.