Evidence map›Paper›PMID 40099899›Full record

ArticlemSphere2025

Assessment of periodontitis vaccine using three different bacterial outer membrane vesicles in canine model.

Ryoma Nakao, Takehiro Yamaguchi, Haruka Shibasaki, Jun Saeki, Aoi Takahashi, Ryunosuke Tominaga, Kimihiro Abe, Yukihiro Akeda, Tomoyo Nakagawa-Nakamura, Tomohiko Nishino and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Virulence · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ryoma NakaoDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.ORCID 0000-0002-4802-8518
Takehiro YamaguchiDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.ORCID 0000-0002-5965-6499
Haruka ShibasakiDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.
Jun SaekiDepartment of Animal Sciences, Teikyo University of Science, Adachi, Tokyo, Japan.ORCID 0009-0004-0725-0835
Aoi TakahashiDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.ORCID 0009-0009-2824-0966
Ryunosuke TominagaDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.
Kimihiro AbeDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.
Yukihiro AkedaDepartment of Bacteriology I, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.
Tomoyo Nakagawa-NakamuraSchool of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji, Tokyo, Japan.ORCID 0000-0003-0858-3661
Tomohiko NishinoSchool of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji, Tokyo, Japan.ORCID 0000-0002-2868-0461
Kazuyuki IshiharaDepartment of Microbiology, Tokyo Dental College, Chiyoda, Tokyo, Japan.ORCID 0000-0002-6567-807X
Atsushi Jinno-OueBioresource Center, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.ORCID 0009-0006-1062-6148
Satoshi InoueDepartment of Veterinary Science, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.ORCID 0000-0002-0691-0000

Funding

Japan Society for the Promotion of Science 20K09943Japan Society for the Promotion of Science 21K18284Japan Society for the Promotion of Science 21KK0164Ministry of Education, Culture, Sports, Science and Technology 20H03861
6 · The paper itself

Abstract

Canines frequently develop periodontitis, which is similar and relevant to immunopathology and microbiology of human periodontitis. The aim of this study was to investigate whether bacterial outer membrane vesicle (OMV)-based periodontal vaccines induced humoral immune response in canines from a human vaccine development perspective. IMPORTANCE: Bacterial outer-membrane vesicles (OMVs) are attractive for use as novel nanoparticle adjuvants, as well as delivery platforms. Periodontal diseases are the most prevalent oral diseases in humans and have serious health and economic burdens, greatly reducing quality of life. The aim of this study is to investigate the humoral immune responses to an OMV-based periodontal disease vaccine in beagles. The vaccine elicited strong mucosal immune responses when administered to beagles by a four-dose heterologous immunization (IN-IN-IN prime and subcutaneous [SC] boost). The OMV vaccine significantly altered the composition of the microbial community in the oral cavity. These findings suggest the utility of the intranasal (IN) prime followed by the SC boost regimen as a rational option to elicit robust humoral immune responses in canines, and most probably in humans as well. We here discuss the outcomes of beagle experiments, the mechanism behind immunological escape of Pg from host immunity, and a rational perspective toward sterilizing immunity in the oral cavity.

Indexed as

Bacterial Outer MembraneBacterial Outer Membrane ProteinsBacterial VaccinesPeriodontitisPorphyromonas gingivalisAdministration, IntranasalAnimalsAntibodies, BacterialDisease Models, AnimalDogsFemaleImmunity, HumoralMiceSalivaTreponema denticolaAntibodies, BacterialBacterial Outer Membrane ProteinsBacterial Vaccinesadjuvantbeagle modelouter membrane vesicles (OMVs)periodontal diseasePorphyromonas gingivalisTreponema denticola: probiotic Escherichia colivaccines

Identifiers

PMID40099899
PMCPMC12039265

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.