ArticleBMC oral health2025
Comparative analysis of the inflammatory response of human gingival fibroblasts to NeoSEALER Flo and CeraSeal bioceramic sealers: an in vitro study.
Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Comparative in vitro evaluation of sonic, passive ultrasonic, and Er: YAG laser-activated irrigation on Enterococcus faecalis reduction and smear layer/debris removal in root canals.BMC oral health · 2026Article
- Kaempferol conjugated selenium nanoparticles mitigate kidney injury from rhabdomyolysis through antioxidant and anti-inflammatory actions via Nrf2/NF-κb pathway.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Targeting mitochondrial dysfunction and apoptosis in 5-fluorouracil-induced cardiotoxicity using kaempferol-mediated selenium nanoparticles.Scientific reports · 2026Article
- Thermo-mechanical loading of innovative "Mini-skirt" preparation design versus conventional full-coverage maxillary central incisor preparation design: an in vitro study.BMC oral health · 2026Article
- Bioceramic versus conventional endodontic sealers: An umbrella review.Journal of conservative dentistry and endodontics · 2026Review
- Fracture resistance of maxillary lateral incisors: effects of fiber post dimensions, material, and surface treatment.BMC oral health · 2026Article
- EGCG-mediated selenium nanoparticles protect against 5-fluorouracil-induced cardiotoxicity via Nrf2/Keap1 signaling.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Cytotoxicity of Root Canal Sealers and Potential Clinical Implications: A Comprehensive Systematic Review of In Vitro Studies.Journal of clinical medicine · 2026Review
- Nephroprotective Effects of Quercetin-Selenium Nanoparticles Against Glycerol-Induced AKI.International journal of molecular sciences · 2025Article
- Comprehensive Approach of a Traumatized and Discolored Anterior Tooth in Geriatric.Case reports in dentistry · 2025Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTherapeutic quality of endodontic sealers plays a critical role in promoting the success of root canal therapy by blocking entrance of microbes as well as facilitating tissue reparative process. The bioceramic sealers NeoSEALER Flo and CeraSeal have been on the rise owing to their biocompatibility and bioactivity. However, their relations with periapical tissues especially human gingival fibroblasts (HGFs) are still significant determinants of treatment outcomes. This in vitro case intends to analyze the inflammatory reaction of HGFs towards NeoSEALER Flo and CeraSeal bioceramic sealers and AH plus resin sealer as control. MATERIALS AND
methodsHGFs were cultured and treated with eluates of tested sealers at different dilutions; 25%, 50%, 75% and 100%. The extracts were left in incubator for 1, 3, and 7 days. Cell death was determined by the MTT assay, and the secretion levels of pro-inflammatory cytokines such as IL-6, IL-8, and TNF-α, were measured using q RT-PCR. Furthermore, mRNA and protein levels of some inflammatory markers were also estimated by q RT-PCR.
resultsAnalysis of the data showed that NeoSEALER Flo was cytotoxic in a concentration-dependent manner to HGFs, though it appeared marginally more toxic than CeraSeal when tested at the same ratios and same time. The high levels of all the measured pro-inflammatory cytokines in HGFs treated with both sealers at a higher concentration with NeoSEALER Flo showing a more intense effect. Having said so, gene expression profiles endorsed these results by showing that both sealers increase the level of IL-6, IL-8, and TNF-α at the higher concentrations.
conclusionsThe study indicates that both NeoSEALER Flo and CeraSeal bioceramic sealers activate HGFs inflammatory response with a slight preference for CeraSeal biocompatibility. Therefore, it becomes important and relevant to assess the cytotoxic and inflammatory propensity of various endodontic materials in order to guide enrolment of these endodontic products in clinical practice and improve the quality of endodontic treatment. Subsequent in vivo works should be done to confirm these in vitro findings as well as to examine the chronic effects of sealer-tissue interaction.
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