Evidence map›Paper›PMID 40098159›Full record

ArticleRespiratory research2025

TLR5 activation in respiratory epithelial cells orchestrate mucosal Th17 response through both indirect and direct pathways.

Sijian Huang, Xu Li, Yuan Cao, Man Mou, Jianlun Li, Kexing Zhuo, Lijuan Wang, Zihang Zeng, Xianghong Wei, Chunlian Tang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Respiratory research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sijian Huang *Institute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Xu Li *Institute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Yuan CaoInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Man MouInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Jianlun LiInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Kexing ZhuoInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Lijuan WangInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Zihang ZengInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Xianghong WeiInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China.
Chunlian TangInstitute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China. tangchunlina@sina.com.
Maohua Zhong *Institute of Infection, Immunology and Tumor Microenvironment, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, # 2 Huangjiahu West Road, Wuhan, Hubei, 430065, China. zmh@wust.edu.cn.

Funding

Innovation research Fund of Wuchang Hospital WCYY2022G01"The 14th Five Year Plan" Hubei Provincial advantaged characteristics disciplines (groups) project of Wuhan University of Science and Technology 2023C0307the National Natural Science Foundation of China 81971568, 81302609 and 82070810the Scientific research plan of Education Department of Hubei Province B2023016
6 · The paper itself

Abstract

backgroundFlagellin, a potent mucosal adjuvant administered via the intranasal route, has been widely recognized for its capacity to enhance immune responses against diverse pathogens. However, the effects and the underlying mechanisms by which flagellin modulates CD4

methodsRecombinant flagellin proteins, including full-length flagellin (SF) and a TLR5-binding deficient variant (SFΔ90-97), were produced and purified. An OT-II derived CD4

resultsFlagellin preferentially promotes Th17 cells differentiation. Respiratory epithelial cells (RECs), acting as sentinel cells, are the first to encounter exogenous stimuli during intranasal immunization. Flagellin stimulates the secretion of various soluble cytokines by binding to TLR5 on the surface of RECs, with GM-CSF facilitating the functional activation of airway DCs. GM-CSF-conditioned DCs exhibit upregulated IL-6 expression which in turn drives the polarization of naïve CD4

conclusionFlagellin preferentially induced a Th17-enhanced immune response and RECs were highlighted its essential roles during this process through both indirect and direct pathways. For indirect pathway, RECs modulate DC function through GM-CSF. Moreover, RECs directly contribute to Th17 differentiation by secreting IL-6.

Indexed as

Epithelial CellsImmunity, MucosalRespiratory MucosaTh17 CellsToll-Like Receptor 5AnimalsCell DifferentiationCells, CulturedDendritic CellsFemaleFlagellinMiceMice, Inbred C57BLSignal TransductionFlagellinTlr5 protein, mouseToll-Like Receptor 5Dendritic cellsFlagellinMucosal adjuvantRespiratory epithelial cellTh17

Identifiers

PMID40098159
PMCPMC11916947

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.