Evidence map›Paper›PMID 40097724›Full record

ArticleCommunications biology2025

Smooth muscle cell Piezo1 depletion results in impaired contractile properties in murine small bowel.

Geoanna M Bautista, Yingjie Du, Michael J Matthews, Allison M Flores, Nicole R Kushnir, Nicolle K Sweeney, Nam Phuong N Nguyen, Elmira Tokhtaeva, R S Solorzano-Vargas, Michael Lewis and 4 more

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Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
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  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Geoanna M Bautista *Department of Pediatrics, Division of Neonatology, University of California Davis Children's Hospital, Sacramento, CA, 95817, USA.ORCID http://orcid.org/0000-0003-0245-9272
Yingjie Du *Department of Materials Science and Engineering, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Michael J MatthewsDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.
Allison M FloresDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.
Nicole R KushnirDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0009-0008-9040-3669
Nicolle K SweeneyDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.
Nam Phuong N NguyenDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0003-4299-5643
Elmira TokhtaevaDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.
R S Solorzano-VargasDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA.
Michael LewisDepartment of Pathology, VA Greater Los Angeles Healthcare System, Los Angeles, CA, 90073, USA.
Matthias StelznerDepartment of Surgery, VA Greater Los Angeles Healthcare System, Los Angeles, CA, 90073, USA.
Ximin HeDepartment of Materials Science and Engineering, University of California Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0001-8845-4637
James C Y DunnDivision of Pediatric Surgery, Departments of Surgery and Bioengineering, Stanford University School of Medicine, Stanford, CA, 94305, USA.ORCID http://orcid.org/0000-0002-6826-7065
Martin G MartinDepartment of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children's Hospital and the David Geffen School of Medicine, University of California Los Angeles, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Los Angeles, CA, 90095, USA. mmartin@mednet.ucla.edu.ORCID http://orcid.org/0000-0002-7192-0504

Funding

UC Davis Clinical and Translational Science CenterUL1TR001860 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KENYON, NICHOLAS J., LYLES, COURTNEY REES · 2016 to 2025
$47.3M
Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
COngenital Diarrhea and Enteropathy (PediCODE) Consortium and BioRepositoryRC2DK118640 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI James Richard Goldenring, Izumi Kaji · 2019 to 2026
$14.8M
Institutional Career Development Core (KL2)KL2TR001859 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI HOLMES, JAMES F. · 2016 to 2025
$10.1M
GASTROENTEROLOGYT32DK007180 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JENSEN, DENNIS MICHAEL · 1986 to 2025
$10.0M
Piezo-1 & 2’s role in murine intestinal muscularis cells of the SIP syncytiumR01DK132319 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI James C Dunn, MARTIN G MARTIN · 2023 to 2026
$2.9M
Neurogenin3 and Intestinal FailureR01DK083762 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MARTIN, MARTIN G · 2009 to 2012
$1.6M
Intestinal Tissue EngineeringR01DK083319 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUNN, JAMES C · 2010 to 2012
$998k
American Academy of Pediatrics (AAP) Marshall Klaus 20202900California Institute for Regenerative Medicine (CIRM) TB1-01183NCATS NIH HHS KL2 TR001859NCATS NIH HHS UL1 TR001860NIDDK NIH HHS P30 DK041301NIDDK NIH HHS R01 DK083319NIDDK NIH HHS R01 DK083762NIDDK NIH HHS R01 DK132319NIDDK NIH HHS RC2 DK118640NIDDK NIH HHS T32 DK007180U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) P30 DK41301
6 · The paper itself

Abstract

Piezo1 is a mechanosensitive cation channel expressed in intestinal muscularis cells (IMCs), including smooth muscle cells (SMCs), interstitial cells of Cajal, and Pdgfrα+ cells, which form the SIP syncytium, crucial for GI contractility. Here, we investigate the effects of SMC-specific Piezo1 deletion on small bowel function. Piezo1 depletion results in weight loss, delayed GI transit, muscularis thinning, and decreased SMCs. Ex vivo analyses demonstrated impaired contractile strength and tone, while in vitro studies using IMC co-cultures show dysrhythmic Ca

Indexed as

Gastrointestinal MotilityIntestine, SmallIon ChannelsMuscle ContractionMyocytes, Smooth MuscleAnimalsCalciumCalcium SignalingMaleMiceMice, Inbred C57BLMice, KnockoutCalciumIon ChannelsPiezo1 protein, mouse

Identifiers

PMID40097724
PMCPMC11914552

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.