ArticleNature nanotechnology2025
Genetic encoding and expression of RNA origami cytoskeletons in synthetic cells.
Article in Nature nanotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Devising a divisome for synthetic cells.Nature reviews. Chemistry · 2026Review
- Design of Fuel-Dependent, Complex-Coacervate-Based Synthetic Cells.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Programmable Edge-to-Edge Assembly of RNA Nanostructures.ACS nano · 2026Article
- Programmed membrane breaching with nanosyringes unlocks control over biochemical responses in synthetic cells.Nature nanotechnology · 2026Article
- From Blueprint to Breakthrough: How Far Can We Fold DNA Origami for Nano-Enabled Technologies?JACS Au · 2026Review
- Nanofluidics for Pioneering Synthetic Biology of Bottom-Up Cell-Free Molecular Systems.ACS synthetic biology · 2026Review
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- A synthetic cell microreactor with two types of interacting dynamic DNA-based pores.Nature chemistry · 2026Article
- Photoactivated Signaling Networks using DNA-Based Synthetic Organelles as Biomimetic Protocells.Angewandte Chemie (International ed. in English) · 2026Article
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Self-Assembling RNA Nanostructures are Highly Sensitive to Environmental Conditions.Journal of molecular biology · 2026Article
- Programming DNA machines to move.Nature reviews. Chemistry · 2026Review
- Designer RNA nanostructures co-transcribed and self-assembled inside human cell nuclei.Nature communications · 2025Article
- Functions and applications of enzymes in nucleic acid nanotechnology.Journal of nanobiotechnology · 2025Review
- PyFuRNAce: an integrated design engine for RNA origami.Nature communications · 2025Article
- The secret life of RNA and lipids.RNA biology · 2025Review
- Acoustically seeded fabrication of a DNA tesseract into a conductive wire.Nucleic acids research · 2025Article
- Photoactivatable Synthetic Exosomes for RNA-Based Communication Between Artificial Cells and Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Cation-Controlled Assembly, Activity, and Organization of Biomimetic DNA Receptors in Synthetic Cell Membranes.Journal of the American Chemical Society · 2025Article
- Building a Synthetic Cell Together.Nature communications · 2025Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bottom-up synthetic biology seeks to engineer a cell from molecular building blocks. Using DNA nanotechnology, building blocks, such as cytoskeletons, have been reverse-engineered. However, DNA nanostructures rely on chemical synthesis and thermal annealing, and therefore synthetic cells cannot produce them from their constituents such as nucleotides. Here we introduce RNA origami cytoskeleton mimics as alternative nucleic acid-based molecular hardware for synthetic cells, which we express directly inside giant unilamellar lipid vesicles (GUVs) containing a DNA template and a polymerase, chemically fuelled by feeding nucleotides from the outside. We designed RNA origami tiles that fold upon transcription and self-assemble into micrometre-long, three-dimensional RNA origami nanotubes under isothermal conditions. We observe that sequence mutations on the DNA template lead to RNA origami nanotubes and closed-ring phenotypes. Molecular dynamics simulations show that these phenotypic transitions are governed by alterations in the stability of RNA secondary structures. In addition, we achieve cortex formation with aptamer-functionalized RNA nanotubes and show that nanotube polymerization leads to membrane deformation. Altogether, our data suggest that the expression of RNA origami-based hardware will help to explore active, evolvable and RNA-based synthetic cells.
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