Evidence map›Paper›PMID 40097593›Full record

ArticleScientific reports2025

MB based RT-qPCR increase the clinical application of cfEBV DNA for NPC in non-endemic area of China.

Qiao He, Yi Tuo, Yi Zhou, Yue Yan, Xin Liu, Dan Zhao, Qiuju Wang, Hao Luo, Zhengyao Zhang, Fanping Meng and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Qiao HeDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Yi TuoDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Yi ZhouDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China.
Yue YanDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Xin LiuCollege of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Dan ZhaoDepartment of Clinical Laboratory, Chong Qing University Three Gorges Hospital, Chongqing, 404000, China.
Qiuju WangDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Hao LuoDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Zhengyao ZhangDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Fanping MengDepartment of Clinical Laboratory, Chong Qing University Three Gorges Hospital, Chongqing, 404000, China.
Binwu YingDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China.
Dongsheng WangDepartment of Clinical Laboratory, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China.
Mu YangDepartment of Experimental Research, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China. mu.yang@uestc.edu.cn.
Yecai HuangDepartment of radiation oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China. cbyhyc.good@163.com.

Funding

Natural Science Foundation of Sichuan Province of China 23NSFSC1446
6 · The paper itself

Abstract

To compare the performance of magnetic bead (MB) and the concentrated precipitation (CP) based RT-qPCR to qualify cell free EBV DNA (cfEBV DNA) for nasopharyngeal carcinoma (NPC) in non-endemic area of China. From January 2014 to June 2024, a retrospective analysis of 2 cohort studies on cfEBV DNA in NPC patients was conducted to assess the diagnostic value, positive detection rate and clinical application. cfEBV DNA detection with CP based RT-qPCR in cohort 1 and MB based RT-qPCR method in cohort 2. The MB based RT-qPCR for the quantitative measurement of cfEBV DNA load was higher than the CP based RT-qPCR in the same plasma samples from NPC patients (P < 0.001). CP based RT-qPCR measured cfEBV DNA in 1405 NPC and 244 healthy controls in cohort 1 with 40.8% sensitivity (AUC = 0.704, 95% CI: 0.676-0.731). In cohort 2(683 naive NPC and 303 controls), cfEBV DNA had a sensitivity of 75.84% (AUC = 0.879, 95% CI: 0.86-0.90). There were no significant differences in TNM stage among NPC between the two cohorts (P > 0.05). The MB method considerably increased the positive detection rate of cfEBV DNA in NPCs at stages III-IV, T2-T4, N1-N3, and M0 (P < 0.001). At the end of treatment, 97.51% of patients had no detectable EBV and just 2.49% had detectable cfEBV DNA. Those who received ≤ 2 or ≥ 3 cycles of NAC had a median t

Indexed as

Cell-Free Nucleic AcidsDNA, ViralEpstein-Barr Virus InfectionsHerpesvirus 4, HumanNasopharyngeal CarcinomaNasopharyngeal NeoplasmsReal-Time Polymerase Chain ReactionAdultAgedChinaFemaleHumansMaleMiddle AgedRetrospective StudiesSensitivity and SpecificityCell-Free Nucleic AcidsDNA, ViralcfEBV DNACPMBNPCRT-qPCR

Identifiers

PMID40097593
PMCPMC11914554

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.