ArticleThe Plant cell2025
Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early PSII assembly factor.
Article in The Plant cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Complexome profiling of mitochondria in the myzozoan parasite of oysters, Perkinsus marinus.PLoS pathogens · 2026Article
- Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme.Nature communications · 2026Article
- APE1 is involved in the stabilization/formation of the RC47 complex of PSII in oxygenic prototrophic organisms.Plant physiology · 2026Article
- Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants.Nature plants · 2025Article
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20 authors.
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Abstract
PSII assembly requires auxiliary factors, including Psb28. Although the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas (Chlamydomonas reinhardtii) psb28 null mutant is severely impaired in PSII assembly, showing drastically reduced PSII supercomplexes, dimers, and monomers, while overaccumulating early PSII assembly intermediates reaction center II (RCII), CP43mod, and D1mod. The mutant had less PSI and more cytochrome b6f complex, its thylakoids were organized mainly as monolayers, and it had a distorted chloroplast morphology. Complexome profiling of the psb28 mutant revealed that THYLAKOID ENRICHED FRACTION 5 (TEF5), the homolog of Arabidopsis (Arabidopsis thaliana) PHOTOSYSTEM B PROTEIN 33/LIGHT HARVESTING-LIKE 8, comigrated particularly with RCII. TEF5 also interacted with PSI. A Chlamydomonas tef5 null mutant was severely impaired in PSII assembly and overaccumulated RCII and CP43mod. RC47 was not detectable in the light-grown tef5 mutant. Our data suggest a possible role for TEF5 in RCII photoprotection or maturation. Both the psb28 and tef5 mutants exhibited decreased synthesis of CP47 and PsbH, suggesting negative feedback regulation possibly exerted by the accumulation of RCII and/or CP43mod in both mutants. The strong effects of missing auxiliary factors on PSII assembly in Chlamydomonas suggest a more effective protein quality control system in this alga than in land plants and cyanobacteria.
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