ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2025
OMIP-111: Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Discovery of a small molecule TLR3 agonist adjuvant.Nature communications · 2025Article
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9 authors.
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Abstract
Functional cytokines shape both innate and adaptive immune responses in the host after infection or immunization. Deep immunophenotyping of the key functional cytokine signatures associated with T cells in murine lymphoid tissue, especially in the spleen, is challenging. Using spectral flow cytometry, we developed a 17-parameter panel to profile major immune cell subsets along with T cells, memory phenotypes, and functional cytokines in murine splenocytes in steady state as well as in stimulated conditions. This panel dissects the memory T cell compartment via CD62L and CD44 expression after mitogen stimulation. To profile T helper (Th) cell distribution after mitogen stimulation, established Th1 markers IFNγ, TNF, and IL-2; Th2 markers IL-4/5; and the Th17 marker, IL-17, are included. This optimized multicolor spectral flow panel allows a detailed immune-profiling of functional cytokines in the murine T cell compartment and might be useful for exploratory analysis of how these functional cytokines shape host immunity after infection or vaccination. Our panel could be easily modified if researchers wish to tailor the panel to their specific needs.
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