Evidence map›Paper›PMID 40095194›Full record

ArticleMikrochimica acta2025

Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells.

Chao Guo, Tongnian Gu, Shao-Hua Wen, Yuan Dang, Yuanzhen Zhou, Junping Ma, Sha Yu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chao Guo *School of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, P.R. China.
Tongnian Gu *Sunresin New Materials Co. Ltd., Xi'an Hi-tech Industrial Development Zone, Xi'an, 710076, P.R. China.
Shao-Hua WenSchool of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, P.R. China.
Yuan DangSchool of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, P.R. China.
Yuanzhen ZhouSchool of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, P.R. China.
Junping MaSchool of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, P.R. China. mjp@xauat.edu.cn.
Sha YuSchool of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, P.R. China. shayu@xauat.edu.cn.

Funding

National Natural Science Foundation of China 22104118National Natural Science Foundation of China 22274125Shaanxi Fundamental Science Research Project for Chemistry and Biology 23JHQ006Young Star of Science and Technology of Shaanxi Province 2023KJXX-146Young Talent Support Project of Shaanxi Science and Technology Association 20240619
6 · The paper itself

Abstract

An intramolecular enhanced entropy-driven DNA amplifier-tethered gold nanoparticle (DNA-Au) nanodevice has been designed for highly sensitive in situ imaging of messenger ribonucleic acid (mRNA) in living cells. The DNA amplifier is immobilized on a same AuNP and the initial fluorescence of DNA-Au nanodevice is quenched. Upon internalized into the target cancer cells, the nanodevice can be activated by endogenous TK1 mRNA, and promptly release the fluorophore via the intramolecular enhanced DNA strand displacement reaction. The decreasing distance and increasing local concentration of the probes via intramolecular reaction can significantly improve the reaction kinetics of DNA-Au nanodevice, thus achieving the highly sensitive imaging of TK1 mRNA. The excellent sensitivity and selectivity allow the DNA-Au nanodevice to accurately discriminate different cell lines and monitor the variations in intracellular TK1 mRNA expression levels via fluorescence imaging. Therefore, the proposed intramolecular enhanced entropy-driven DNA-Au nanodevice will afford a reliable approach for accurate determination of mRNA in molecular diagnostic systems.

Indexed as

DNAGoldMetal NanoparticlesOptical ImagingRNA, MessengerEntropyFluorescent DyesHeLa CellsHumansThymidine KinaseDNAFluorescent DyesGoldRNA, MessengerThymidine Kinasethymidine kinase 1DNA-Au nanodeviceEntropy-driven DNA amplifierIntramolecular reactionmRNA imaging

Identifiers

PMID40095194

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.