Evidence map›Paper›PMID 40092609›Full record

ArticleiScience2025

Spatiotemporal profiling of protein kinase A activity in spontaneously beating hiPSC-derived cardiac organoids.

Ido Weiser-Bitoun, Savyon Mazgaoker, Rami Eid, Inbar Brosh, Yael Yaniv

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In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ido Weiser-BitounLaboratory of Bioelectric and Bioenergetic Systems, Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Savyon MazgaokerLaboratory of Bioelectric and Bioenergetic Systems, Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Rami EidLaboratory of Bioelectric and Bioenergetic Systems, Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Inbar BroshLaboratory of Bioelectric and Bioenergetic Systems, Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Yael YanivLaboratory of Bioelectric and Bioenergetic Systems, Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein kinase A (PKA) phosphorylates proteins crucial for rhythm modulation, with its dysregulation linked to arrhythmias. This study investigated PKA activity's spatiotemporal dynamics in spontaneously beating cardiac organoids. We hypothesized that PKA activity would respond to autonomic stimulation and exhibit spatial heterogeneity upon drug-induced modulation. Forskolin (activator) and H-89 (inhibitor) altered PKA activity ratios from 1 to 1.52 ± 0.03 and 0.89 ± 0.03, respectively. Hill equation-based regression showed a high fit of PKA activity behavior for all four tested drugs (forskolin, H-89, isoproterenol, or carbachol) at concentrations. Responses to forskolin or isoproterenol, which increase PKA activity, showed higher heterogeneity (10.1 ± 0.8% or 8.7 ± 1%) compared to responses to H-89 or carbachol (4.3 ± 0.8% or 4.4 ± 1.2%), which decrease PKA activity. These results reveal the intricate spatial dynamics of PKA activity in cardiac organoids and its dependence on PKA activation levels.

Indexed as

Biological sciencesCell biologyNatural sciencesStem cells research

Identifiers

PMID40092609
PMCPMC11910075

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.