ArticleOpen veterinary journal2025
Application of real-time PCR for analysis canine meat (
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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9 authors.
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Abstract
Background: Canine meat (CM) is one of the non-halal meats prohibited for consumption by the Muslim community. Due to its low prices compared with beef, CM is typically used as meat adulterants in halal food-based products such as Satay and meatballs to get economic profits. Aim: The objective of this study was to design a novel species-specific primer in combination with real-time polymerase chain reaction for analysis of Canine's DNA for halal authentication analysis. Methods: A Primer targeting the D-loop region of mitochondrial DNA was designed and subjected to a validation procedure by assessing some performance characteristics including specificity, amplification efficiency (E), sensitivity, repeatability, and linearity describing the correlation between the concentration of Canine's DNA ( Results: The Real-Time Polymerase Chain Reaction (RT-PCR) method produced an acceptable amplification efficiency (E) of 109.7% with the coefficient of determination ( Conclusion: RT-PCR combined with a novel primer targeting D-loop provides a specific and accurate analytical tool for the identification of CM for halal authentication studies.
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