Evidence map›Paper›PMID 40092159›Full record

ArticleSynthetic and systems biotechnology2025

A dual light-controlled co-culture system enables the regulation of population composition.

Wei Jiang, Yijian Guo, Xuanshuo Liang, Ying Zhang, Jianning Kang, Zhengxin Jin, Bin Ning

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wei JiangCentral Hospital Affiliated to Shandong First Medical University, Jinan, 250013, Shandong, PR China.
Yijian GuoJinan Central Hospital, Shandong University, Jinan, 250013, Shandong, PR China.
Xuanshuo LiangWest China Medical Center, Sichuan University, Chengdu, 610041, Sichuan, PR China.
Ying ZhangCentral Hospital Affiliated to Shandong First Medical University, Jinan, 250013, Shandong, PR China.
Jianning KangJinan Central Hospital, Shandong University, Jinan, 250013, Shandong, PR China.
Zhengxin JinJinan Central Hospital, Shandong University, Jinan, 250013, Shandong, PR China.
Bin NingCentral Hospital Affiliated to Shandong First Medical University, Jinan, 250013, Shandong, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With the development of metabolic engineering, increasing requirements for efficient microbial biosynthesis call for establishment of multi-strain co-culture system. Dynamic regulation of population ratios is crucial for optimizing bioproduction performance. Optogenetic systems with high universality and flexibility have the potential to realize dynamic control of population proportion. In this study, we utilized an optimized chromatic acclimation sensor/regulator (CcaS/R) system and a blue light-activated YF1-FixJ-PhlF system as induction modules. A pair of orthogonal quorum sensing systems and a toxin-antitoxin system were employed as communication module and effector module, respectively. By integrating these modules, we developed a dual light-controlled co-culture system that enables dynamic regulation of population ratios. This co-culture system provides a universal toolkit for applications in metabolic engineering and synthetic biology.

Indexed as

Co-culture systemDual light-controlled systemOptogenetic switchPopulation ratio regulation

Identifiers

PMID40092159
PMCPMC11910626

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.