ArticleMicrobial cell factories2025
Acetol biosynthesis enables NADPH balance during nitrogen limitation in engineered Escherichia coli.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Multi-Omics Reveals the Impact of Domestic Wastewater Input on the Dissolved Organic Carbon Pool and Microbial Community in the Qiantang River Estuary.Microorganisms · 2026Article
- Advances in biotechnological methods for genetic and metabolic engineering in Methylomonas sp. DH-1.Journal of biological engineering · 2026Review
- Analysis of metabolic stability under environmental perturbations using a kinetic model.Biophysics and physicobiology · 2026Article
- Harnessing glycerol for secondary metabolite biosynthesis in microorganisms.World journal of microbiology & biotechnology · 2025Review
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9 authors.
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Abstract
backgroundNutrient limitation strategies are commonly applied in bioprocess development to engineered microorganisms to further maximize the production of the target molecule towards theoretical limits. Biomass formation is often limited under the limitation of key nutrients, and understanding how fluxes in central carbon metabolism are re-routed during the transition from nutrient excess to nutrient-limited condition is vital to target and tailor metabolic engineering strategies. Here, we report the physiology and intracellular flux distribution of an engineered acetol-producing Escherichia coli on glycerol under nitrogen-limited, non-growing production conditions.
resultsAcetol production in the engineered E. coli strain is triggered upon nitrogen depletion. During nitrogen limitation, glycerol uptake decreased, and biomass formation rates ceased. We applied
conclusionThe results reported in this study illustrate how the production of a value-added chemical from a waste stream can be connected to the metabolism of the whole-cell biocatalyst, making product formation mandatory for the cell to maintain its NADPH/NADP
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