ArticleJournal of orthopaedic surgery and research2025
Synergistic influences of BMP9 and NGF on the osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.
Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Zinc sulfate modulates the C3H10T1/2 mesenchymal stem cell line secretome and NFκB signaling during inflammation to influence selected immune cell responses.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Article
- Neuro-immune-vascular-stem cell crosstalk in bone/cartilage regeneration: mechanisms, technological advances, and clinical perspectives.Frontiers in bioengineering and biotechnology · 2026Review
- Upregulation of lncRNA SSTR5-AS1 promotes osteoblast differentiation and reduces apoptosis.Journal of orthopaedic surgery and research · 2025Article
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4 authors.
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Abstract
backgroundBone morphogenetic protein 9 (BMP9) and nerve growth factor (NGF) are critical factors influencing osteogenic differentiation in mesenchymal stem cells (MSCs). While BMP9 has been recognized for its potent osteogenic capabilities, NGF's role in bone tissue engineering is less understood. This investigation delineated the synergistic link of BMP9 with NGF in driving osteogenic differentiation in C3H10T1/2 MSCs.
objectiveTo evaluate the combined impact of BMP9 and NGF on osteogenic markers' expression levels and the formation of calcified nodules in C3H10T1/2 cells, providing a basis for the enhanced bone regeneration strategies in tissue engineering.
methodsC3H10T1/2 cells were subjected to treatment regimens incorporating NGF at variable concentrations (10, 50, and 100 ng/ml) and BMP9, either as monotherapies or in combination. Osteogenic differentiation' comprehensive assessment was undertaken by quantifying early-stage markers (Runx2, Col I) and late-stage markers (OPN) via RT-PCR, Western blotting, ALP staining, and Alizarin Red S staining for mineralized matrix deposition.
resultsNGF elicited a concentration-dependent augmentation of early osteogenic markers, with the 10 ng/ml dosage demonstrating maximal efficacy. BMP9 independently facilitated robust osteogenic differentiation, whereas the combinatorial treatment with BMP9 and NGF synergistically amplified the expression levels of Runx2, Col I, and OPN. Notably, this combined treatment yielded a remarkable enhancement in the deposition of mineralized extracellular matrix, as evidenced by a notable escalation in the size and density of calcified nodules relative to monotherapies.
conclusionThe findings unveiled the remarkable synergistic link of BMP9 with NGF in potentiating osteogenic differentiation in C3H10T1/2 MSCs. This dual-factor approach presents a compelling paradigm for advancing bone regeneration strategies, providing substantial promise for utilization in bone tissue engineering plus regenerative medicine.
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