ArticleScientific reports2025
RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Old nodes, new tricks: optimized methods for chronic wasting disease prion detection in preserved retropharyngeal lymph nodes.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
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Authors and funding
8 authors.
Funding
Abstract
Chronic wasting disease (CWD) is a fatal neurodegenerative disease in cervids which is caused by prions, and new cases continue to appear in populations in North America and globally. The United States Department of Agriculture-approved tests for diagnosing CWD use obex and/or retropharyngeal lymph nodes, which are challenging to collect as the tissues require anatomical knowledge, skill, and time to dissect. Third eyelids contain lymphoid follicles and are easier to collect. We determine whether third eyelids from naturally infected white-tailed deer are a reliable tissue for detecting CWD prions using real-time quaking-induced conversion (RT-QuIC), if positive results can be confirmed by immunohistochemistry, and if the results are reproducible between laboratories. Testing of third eyelids individually by RT-QuIC had a sensitivity of 94% and a specificity of 100%, and pooling of 5 third eyelids into one sample yielded a sensitivity of 100% and a specificity of 100%. Although IHC on third eyelid can be used in conjunction with RT-QuIC to support a positive diagnosis, using IHC on third eyelids in isolation should be performed with caution as additional validation for this tissue type is recommended. Our results support that testing third eyelids is a potentially more efficient way for management agencies to improve and expand their CWD surveillance.
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