Evidence map›Paper›PMID 40089537›Full record

ArticleScientific reports2025

A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli.

James Bosco, Emily Gagliano, Kassandra L Boshae, John P Statz, Timothy B Wheeler, DeAnna Cuello, Ashlyn Sliter, Christian Newby, Bernice Lin, Aysha Demeler and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

James BoscoDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA. james.bosco@umontana.edu.
Emily GaglianoDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Kassandra L BoshaeDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
John P StatzDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Timothy B WheelerDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
DeAnna CuelloDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Ashlyn SliterDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Christian NewbyDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Bernice LinDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Aysha DemelerDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, Lethbridge, AB, T1K 3M4, Canada.
C Logan PierpontDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Cindee Yates-HansenDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Matthew J SydorDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Maria E FerriniDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Kellie C KuchDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Brandon S CooperDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Beverly J PiggottDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Sarah J CertelDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Kasper B HansenDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Stephen R SprangDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Bruce BowlerDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Levi McClellandDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA.
Mehmet BerkmenNew England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.
Ekaterina VoroninaDivision of Biological Sciences, Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, University of Montana, Missoula, MT, 59812, USA. ekaterina.voronina@umontana.edu.

Funding

A Synchrotron Radiation Structural Biology ResourcesP30GM133894 · NIGMS · STANFORD UNIVERSITY · PI KEITH O HODGSON · 2020 to 2026
$43.3M
Surveillance genome sequencing to detect SARS-CoV-2 virus variants in MontanaP20GM103546 · NIGMS · UNIVERSITY OF MONTANA · PI BOWLER, BRUCE E · 2012 to 2021
$19.2M
Surveillance genome sequencing to detect SARS-CoV-2 virus variants in MontanaP30GM140963 · NIGMS · UNIVERSITY OF MONTANA · PI BOWLER, BRUCE E · 2021 to 2025
$6.9M
Molecular Histology and Fluorescent ImagingP30GM103338 · NIGMS · UNIVERSITY OF MONTANA · PI WETZEL, SCOTT A · 2013 to 2017
$5.6M
The evolutionary genetics and genomics of Wolbachia effects on host physiologyR35GM124701 · NIGMS · UNIVERSITY OF MONTANA · PI Brandon S. Cooper · 2017 to 2026
$3.9M
Structural and functional investigation of negative allosteric NMDA receptor modulationR01NS097536 · NINDS · UNIVERSITY OF MONTANA · PI Kasper Boe Hansen · 2016 to 2026
$3.4M
P granules and control of germ cells developmentR01GM109053 · NIGMS · UNIVERSITY OF MONTANA · PI VORONINA, EKATERINA · 2014 to 2022
$2.5M
Physiology and pharmacology of GluN3-containing NMDA receptorsR01NS116055 · NINDS · UNIVERSITY OF MONTANA · PI HANSEN, KASPER BOE · 2021 to 2025
$1.8M
Acquisition of a Nikon A1 confocal laser-scanning microscope with PicoQuant FCS & FLIM upgradeS10OD021806 · OD · UNIVERSITY OF MONTANA · PI ROSS, J B ALEXANDER · 2016 to 2016
$555k
National Science Foundation CHE-1904895NIGMS NIH HHS P20 GM103546NIGMS NIH HHS P30 GM103338NIGMS NIH HHS P30 GM133894NIGMS NIH HHS P30 GM140963NIGMS NIH HHS R01 GM109053NIGMS NIH HHS R35 GM124701NIH HHS P30GM140963NIH HHS R01GM109053NIH HHS R01NS097536NIH HHS S10 OD021806NINDS NIH HHS R01 NS097536NINDS NIH HHS R01 NS116055
6 · The paper itself

Abstract

Protein production using Escherichia coli is a cornerstone of modern biotechnology. In this study, we developed a novel auto-expression medium to maximize protein production. Each E. coli strain tested was capable of auto-expression in response to galactose, including strains in which the endogenous lacZ had been disrupted. This provides key evidence that galactose can regulate the lac operon independent of known lac operon-regulated metabolism. The enhanced capabilities of the novel auto-expression medium were documented across protein production systems including (1) increased yields for routinely expressed proteins (e.g. eGFP), (2) improved expression of human cytochrome c within a dual expression system, (3) robust auto-expression in lacZ-deficient strains producing proteins with challenging disulfide bonds, and (4) reproducible 8-fold increase in SpCas9 yields, at ≥ 95% purity. This novel medium can streamline production and improve yields for routine as well as challenging proteins, accelerating recombinant protein production and creating new opportunities in biotechnology and structural biology.

Indexed as

Escherichia coliGalactoseRecombinant ProteinsGene Expression Regulation, BacterialGreen Fluorescent ProteinsHumansLac OperonGalactoseGreen Fluorescent ProteinsRecombinant Proteins

Identifiers

PMID40089537
PMCPMC11910606

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.