Evidence map›Paper›PMID 40088373›Full record

ArticleAMB Express2025

Treatment of Staphylococcus aureus-infected diabetic wounds by melatonin loaded nanocarriers.

Alyaa Farid, Ayah Mohsen, Bassant Nasser, Habiba Alaa, Mariam Abdelaziz, Maryam Mustafa, Mustafa Mansour, Nourhan Adel, Salma Magdy, Salma Mohsen and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Alyaa FaridBiotechnology Department, Faculty of Science, Cairo University, Giza, Egypt. alyaafarid@cu.edu.eg.ORCID http://orcid.org/0000-0003-0706-347X
Ayah MohsenBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Bassant NasserBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Habiba AlaaBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.
Mariam AbdelazizBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.
Maryam MustafaBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.
Mustafa MansourBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.
Nourhan AdelBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Salma MagdyBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.
Salma MohsenBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.
Samah AdelBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Sarah IbrahimBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Shaymaa Abdel-RahmanBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Sohaila MohamedBiochemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Yomna El-KaramanyBiotechnology/Biomolecular chemistry program, Faculty of Science, Cairo University, Giza, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the complication of diabetes mellitus is chronic wounds. The healing of wounds in diabetic patients is retarded by the elevation in the pro-inflammatory cytokines secretion and free radicles accumulation. Wound management in diabetic patients requires preventing bacterial biofilm development. Due to the wound healing activity of chitosan (CS), lecithin (Le) and melatonin (M), the present study aimed to load melatonin on CS/Le NPs and examine their effect on diabetic wounds infected with Staphylococcus aureus. Melatonin loaded chitosan/lecithin nanoparticles (M-CS/Le NPs) were physically characterized and their antioxidant, anti-inflammatory and antimicrobial activities were examined in vitro. Male Sprague Dawley rats included two division (non-diabetic and diabetic) which were further divided in nine groups. Diabetes induction and follow up throughout the experimental period was confirmed by measuring the levels of fructosamine and blood glucose. Full-thickness wounds was induced in both non-diabetic and diabetic animals followed by infection with Staphylococcus aureus according to the experimental design. The wound healing effect of M-CS/Le NPs was evaluated through measurements of the oxidative stress, inflammatory cytokines and apoptotic proteins. Our results showed the anti-microbial, free radical scavenging and hemolysis inhibition effects of M-CS/Le NPs in vitro. Moreover, the preparation of M-CS/Le NPs decreased the dose of used melatonin (when compared to free melatonin). M-CS/Le NPs significantly decreased the wound area percent in treated infected wounds of both non-diabetic and diabetic rats more than free melatonin or unloaded CS/Le NPs. In conclusion, M-CS/Le NPs promoted the wound healing in Staphylococcus aureus-infected wounds in diabetic rats.

Indexed as

ChitosanDiabetes mellitusLecithinMelatoninStaphylococcus aureus

Identifiers

PMID40088373
PMCPMC11910460

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.