Evidence map›Paper›PMID 40088352›Full record

ArticleProbiotics and antimicrobial proteins2026

Fermentation of Sargassum ilicifolium with Probiotics (Lactobacillus plantarum and Saccharomyces cerevisiae) Enhances Growth, Immunity, and Gene Expression in Rainbow Trout Before and After Challenge with Streptococcus iniae.

Majid Khanzadeh, Seyed Hossein Hoseinifar, Metin Yazici, Hien Van Doan

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Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Veterinary and animal science · 2026
    Article
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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

4 authors.

Majid KhanzadehAnimal Biological Product Research Group, Academic Center for Education, Culture and Research (ACECR), Tehran Organization, Tehran, Iran. khanzade@acecr.ac.ir.
Seyed Hossein HoseinifarDepartment of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Metin YaziciFaculty of Marine Sciences and Technology, Iskenderun Technical University, Iskenderun, 31200, Turkey.
Hien Van DoanDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand. hien.d@cmu.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigates the effect of Sargassum ilicifolium algae fermentation using Lactobacillus plantarum and Saccharomyces cerevisiae probiotics on growth, immunity, gene expression, and digestive and antioxidant enzymes before and after challenge with Streptococcus iniae in trout. Trout (50.69 ± 1.33 g) were fed with different percentages of S. ilicifolium (SI) powder over an 8-week trial. The supplements included both unfermented (SI 0.5%, SI 1%, SI 2%) and fermented (FSI 0.5%, FSI 1%, FSI 2%) forms. A significant increase in white blood cells was observed in the group receiving 2% FSI compared to the control group (P < 0.05). All growth factors in trout notably increased with varying levels of fermented and unfermented S. ilicifolium powder compared to the control group (P < 0.05). Biochemical parameters including serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, and alkaline phosphatase exhibited a notable rise before the challenge with S. iniae (P < 0.05). Immune parameters, including total immunoglobulin, lysozyme, complement 3, bactericidal activity, and nitroblue tetrazolium showed increases both before and after the challenge with S. iniae (P < 0.05). Additionally, the expression levels of the insulin-like growth factor-1, interleukin-6, tumor necrosis factor-alpha, and Ghrelin genes showed significant increases both before and after exposure (P < 0.05). Also, antioxidant enzymes and digestive enzymes increased significantly before and after exposure (P < 0.05). All levels of unfermented and fermented, except for SI 0.5%, showed significant increases compared to the control group following the challenge with S. iniae (P < 0.05). Overall, fermented and unfermented S. ilicifolium support growth and enhance immune, antioxidant, and digestive functions in rainbow trout effectively.

Indexed as

Fish DiseasesLactiplantibacillus plantarumOncorhynchus mykissProbioticsSaccharomyces cerevisiaeSargassumStreptococcal InfectionsStreptococcus iniaeAnimal FeedAnimalsFermentationGene ExpressionAntioxidant defenseGene expressionImmunityLactobacillus plantarumSaccharomyces cerevisiaeSargassum ilicifolium

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.