Evidence map›Paper›PMID 40088274›Full record

ArticleArchives of microbiology2025

Immunological assessment of NSFu1: A novel fusion molecule constructed from structural proteins of SARS-CoV-2 for improving COVID-19 antibody detection.

Shaista Arif, Mohsina Akhter, Aqsa Anwar, Sania Javaid, Zara Ashi, Mohsin Shad, Asad Rahman, Huda Abbas, Fouzia Ashraf, M Waheed Akhtar and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shaista Arif *School of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Mohsina Akhter *School of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Aqsa AnwarSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Sania JavaidSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Zara AshiSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Mohsin ShadSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Asad RahmanSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Huda AbbasMolecular Diagnostic Division, Department of Pathology and Microbiology, Allama Iqbal Medical College, Jinnah Hospital Lahore, Lahore, Pakistan.
Fouzia AshrafMolecular Diagnostic Division, Department of Pathology and Microbiology, Allama Iqbal Medical College, Jinnah Hospital Lahore, Lahore, Pakistan.
M Waheed AkhtarSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan.
Muhammad SajjadSchool of Biological Sciences, University of the Punjab, Quaid-E-Azam Campus, P.O. 54590, Lahore, Pakistan. sajjad.sbs@pu.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The SARS-CoV-2 outbreak has claimed millions of lives and caused significant clinical challenges. The availability of a rapid, cost-effective, and sensitive test to detect antibodies at different stages of COVID-19 is crucial for effective clinical management, epidemiological studies, and public health surveillance. Four novel peptides (SF1, SF2, SF4, SF6) and two multi-epitope fusion proteins (SFu1 and NSFu1) from less variable regions of the spike and nucleocapsid proteins were developed. After detailed in silico structural validation, all the proteins were expressed in E. coli (BL21), purified by Ni

Indexed as

Antibodies, ViralCOVID-19COVID-19 Serological TestingSARS-CoV-2Spike Glycoprotein, CoronavirusAdultCoronavirus Nucleocapsid ProteinsEnzyme-Linked Immunosorbent AssayFemaleHumansMaleMiddle AgedRecombinant Fusion ProteinsSensitivity and SpecificityAntibodies, ViralCoronavirus Nucleocapsid ProteinsRecombinant Fusion ProteinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2COVID-19Fusion moleculesIgG antibody detectionSensitivity

Identifiers

PMID40088274

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.