Evidence map›Paper›PMID 40087215›Full record

ArticleTransgenic research2025

An advanced cytosine base editor enabled the generation of cattle with a stop codon in the β-lactoglobulin gene.

Qiang Ding, Zhaokang Cui, Qianqian Shi, Yan Zhang, Nan He, Rihong Guo, Yu Tian, Shaoxian Cao, Jifeng Zhong, Huili Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Efficient and Safe Knockout ofVeterinary sciences · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qiang Ding *Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Zhaokang Cui *Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Qianqian ShiKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Yan ZhangShandong OX Livestock Breeding Co., Ltd., Jinan, 250100, China.
Nan HeCollege of Animal Science and Technology, Shandong Agricultural University, Taian, 271017, China.
Rihong GuoKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Yu TianJiangsu Youyuan Dairy Research Institute Co., Ltd., Nanjing, 211100, China.
Shaoxian CaoKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Jifeng ZhongKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Huili WangKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Jiangsu Province Engineering Research Center for Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. wanghuili@jaas.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

β-Lactoglobulin (BLG) is an allergen present in milk that can induce an acute immune response in certain individuals. The successful use of cytosine base editors (CBEs) can introduce stop codons into premature mRNA, thereby generating animals with disrupted genes that negatively regulate target traits. In this study, we employed a CBE system to target the major milk allergen BLG in bovine embryos, mammary epithelial cells, and live cattle. First, the precise single-base editing of the BLG gene in bovine embryos was achieved by designing an effective sgRNA to induce a c.61C > T substitution in the coding region, converting codon 21Gln (p.21Gln) to a premature stop codon. Sanger sequencing revealed an editing efficiency of 83.3% (20 out of 24 embryos), including two homozygous edits. Second, a bovine mammary epithelial cell line harboring BLG edits was constructed using the same CBE system. Sequencing showed that the designed sgRNA1 enabled the simultaneous conversion of three consecutive cytosines (c.59-61CCC > TTT) to thymines. At position c.61, single-cell clones exhibited monoallelic or biallelic editing (BLG

Indexed as

Codon, TerminatorCytosineGene EditingLactoglobulinsAnimalsAnimals, Genetically ModifiedCattleEpithelial CellsFemaleMammary Glands, AnimalMilkCodon, TerminatorCytosineLactoglobulinsBase editingCattleCBEStop codonβ-Lactoglobulin

Identifiers

PMID40087215

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.