Evidence map›Paper›PMID 40085650›Full record

ArticleSTAR protocols2025

Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus.

Olga Vafaeva, Poommaree Namchaiw, Karl Murray, Elva Diaz, Hwai-Jong Cheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Olga VafaevaCenter for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Department of Pharmacology, School of Medicine, University of California, Davis, Davis, CA 95616, USA.
Poommaree NamchaiwCenter for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
Karl MurrayDepartment of Physiology and Membrane Biology, School of Medicine, University of California, Davis, Davis, CA 95616, USA.
Elva DiazDepartment of Pharmacology, School of Medicine, University of California, Davis, Davis, CA 95616, USA. Electronic address: ediaz@ucdavis.edu.
Hwai-Jong ChengCenter for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan. Electronic address: hjcheng@gate.sinica.edu.tw.

Funding

UC Davis MCB T32 Administrative Supplement to Recognize Excellence in Diversity, Equity, Inclusion, and Accessibility (DEIA) MentorshipT32GM007377 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI CHEDIN, FREDERIC LOUIS · 1985 to 2023
$9.9M
SynDIG1/Prrt1 regulation of extrasynaptic GluA1-containing AMPARs during plasticityR01MH119347 · NIMH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI DIAZ, ELVA D, HELL, JOHANNES W · 2019 to 2024
$3.3M
Neuronal Integration of Newborn Granule Cells in Aged BrainsR01AG054649 · NIA · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MURRAY, KARL DANIEL · 2017 to 2021
$1.6M
Proteomic analysis of maturing adult-born hippocampal mossy fiber boutonsR21NS115092 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MURRAY, KARL DANIEL · 2019 to 2020
$432k
NIA NIH HHS R01 AG054649NIGMS NIH HHS T32 GM007377NIMH NIH HHS R01 MH119347NINDS NIH HHS R21 NS115092
6 · The paper itself

Abstract

The neurosphere assay is the gold standard for assessing the proliferative and differentiation capacities of neural progenitor cells (NPCs). Here, we present a protocol for isolating, propagating, and maintaining hippocampal neurospheres from adult and aged mice and differentiating cultured NPCs into neurons and astrocytes. We describe steps for establishing a heterochronic co-culture of neurosphere-derived cells with primary neurons. Using neurospheres from old animals enables investigation of the effects of aging on the development and differentiation of newborn neurons.

Indexed as

AgingCell Culture TechniquesDentate GyrusHippocampusNeural Stem CellsAnimalsCell DifferentiationCells, CulturedCoculture TechniquesMiceNeuronsCell-based AssaysCell cultureCell isolationNeuroscience

Identifiers

PMID40085650
PMCPMC11952782

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.