Evidence map›Paper›PMID 40085274›Full record

ArticleBiotechnology letters2025

Biochemical characterization of a bilfunctional endoglucanase/glucomannanase derived from mountain soil.

Justice Kipkorir Rono, Qingyun Zhang, Yong He, Shaochen Wang, Yunbin Lyu, Zhi Min Yang, Zhiyang Feng

Abstract read
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In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Justice Kipkorir RonoCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Qingyun ZhangCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Yong HeCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Shaochen WangCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Yunbin LyuCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Zhi Min YangDepartment of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Zhiyang FengCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. zfeng@njau.edu.cn.ORCID http://orcid.org/0000-0002-1253-0282

Funding

National Natural Science Foundation of China 32370089
6 · The paper itself

Abstract

Metagenomics is increasingly recognized as a vital technique for exploring uncultured microorganisms, with one key application being the discovery of novel enzymes for industrial use. This study identified an endoglucanase gene from soil metagenome, termed ZFEG1801, which was expressed in E. coli BL21, purified, and characterized for its biochemical properties. The 72.8 kDa recombinant protein exhibited hydrolytic activity against sodium carboxymethyl cellulose (CMC) and konjac glucomannan (KG), with activities of 12.1 U/mg and 42.1 U/mg, respectively. The enzyme displayed optimal activity at pH 5 for CMC and pH 6 for KG, with broad pH stability ranging from 5 to 9. The optimal temperature was 40 °C, and it remained thermally stable between 20 and 40 °C, retaining over 60% of its activity. The enzyme activity remained stable in the presence of most metal ions; however, CMCase activity was inhibited by Cu

Indexed as

CellulaseMannansSoil MicrobiologyCarboxymethylcellulose SodiumCloning, MolecularEnzyme StabilityEscherichia coliHydrogen-Ion ConcentrationHydrolysisKineticsMetagenomeMetagenomicsRecombinant ProteinsSubstrate SpecificityTemperature(1-6)-alpha-glucomannanCarboxymethylcellulose SodiumCellulaseMannansRecombinant ProteinsBifunctional enzymeCarboxymethyl celluloseEndoglucanaseGH5 familyGlucomannanase

Identifiers

PMID40085274

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.