Evidence map›Paper›PMID 40085269›Full record

ArticleBiotechnology letters2025

Metabolic engineering of Escherichia coli for enhanced production of hyaluronic acid.

Syafira Rizqi Eskasalam, Selim Ashoor, Hyeon Jeong Seong, Yu-Sin Jang

Abstract read
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In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. EngineeringBiomolecules · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Syafira Rizqi Eskasalam *Division of Applied Life Science (BK21 Four), Department of Applied Life Chemistry, Institute of Agriculture & Life Science (IALS), Gyeongsang National University (GNU), Jinju, 52828, Republic of Korea.
Selim Ashoor *Division of Applied Life Science (BK21 Four), Department of Applied Life Chemistry, Institute of Agriculture & Life Science (IALS), Gyeongsang National University (GNU), Jinju, 52828, Republic of Korea.
Hyeon Jeong SeongDivision of Applied Life Science (BK21 Four), Department of Applied Life Chemistry, Institute of Agriculture & Life Science (IALS), Gyeongsang National University (GNU), Jinju, 52828, Republic of Korea.
Yu-Sin JangDivision of Applied Life Science (BK21 Four), Department of Applied Life Chemistry, Institute of Agriculture & Life Science (IALS), Gyeongsang National University (GNU), Jinju, 52828, Republic of Korea. jangys@gnu.ac.kr.ORCID http://orcid.org/0000-0003-2044-0479

Funding

Ministry of Science and ICT, South Korea 2022R1A2C1006214Ministry of Science and ICT, South Korea RS-2024-00439872
6 · The paper itself

Abstract

objectivesTo enhance hyaluronic acid (HA) production in Escherichia coli by utilizing hasA genes from Streptococcus pyogenes and Streptococcus parauberis, and employing metabolic engineering strategies.

resultsThe expression of the hasA (SpaHasA) gene from S. parauberis in E. coli K12 W3110 led to higher HA production compared to the other gene. Knockout of the zwf and pfkA genes in the engineered E. coli expressing SpaHasA gene, further increased HA production to 891 mg l

conclusionsThe hasA genes from various Streptococcus groups, especially S. parauberis, significantly boost HA production in E. coli, demonstrating their potential for microbial fermentation applications.

Indexed as

Escherichia coliHyaluronic AcidMetabolic EngineeringBacterial ProteinsCulture MediaFermentationStreptococcusStreptococcus pyogenesBacterial ProteinsCulture MediaHyaluronic AcidEscherichia coliFermentationhasAHyaluronic acidMetabolic engineering

Identifiers

PMID40085269

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.