ArticleChemistry (Weinheim an der Bergstrasse, Germany)2025
Frosted DNA: β-Galactosidase Control of Oligonucleotide Activity.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Enzyme-Responsive Cyclic Gapmer Antisense Oligonucleotides via ThymidineBioconjugate chemistry · 2026Article
- Control of Tetrazine Bioorthogonal Reactivity by Rotaxanation.Angewandte Chemie (International ed. in English) · 2025Article
- Topological Switch-OFF and β-Galactosidase-Triggered Switch-ON of Cyclic Antisense Oligonucleotides via CuAAC for Controlled RNA Cleavage.Bioconjugate chemistry · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We introduce a novel approach for the control of oligonucleotides through enzymatic activation with β-galactosidase (β-gal). We use the well-known enzymatic capability of β-gal to hydrolyze the β-galactosidic bond combined with a self-immolative linker and present three ways of steric or topological blocking of a DNA oligonucleotide. Through a series of in vitro experiments with β-gal variants (recombinant or from human cell lysates), we systematically investigate stability, transitory perturbation, and enzymatic activation. Our approach holds significant promise for applications related to senescence-associated β-gal activity, including targeted modulation of gene expression and programmable molecular interventions. The combination of enzymatic activation applied to oligonucleotides represents a significant advance for targeted release in affected cells without the need for external triggering.
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Registered trials
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