Evidence map›Paper›PMID 40084075›Full record

ArticleBio-protocol2025

Colorimetric Determination of Tungsten and Molybdenum in Biological Samples.

Aaron P Donaghy, Gerrit J Schut, Nana Shao, Farris L Poole, Michael W W Adams

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Aaron P DonaghyDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Gerrit J SchutDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Nana ShaoDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Farris L PooleDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Michael W W AdamsDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.

Funding

W-Health: Tungsten is an Essential Metal for a Healthy Gut MicrobiomeR01GM136885 · NIGMS · UNIVERSITY OF GEORGIA · PI ADAMS, MICHAEL W. · 2020 to 2023
$2.0M
NIGMS NIH HHS R01 GM136885
6 · The paper itself

Abstract

Molybdenum (Mo) and tungsten (W) are elements that are utilized in biological systems. They are typically incorporated into the catalytic sites of enzymes coordinated to an organic pyranopterin cofactor; Mo may also be present in the form of a FeMo cofactor. While Mo is used by all branches of life, only a few microbes are able to utilize W. In order to study Mo- and W-dependent enzymes, it is important to be able to measure Mo and W in biological samples. Methods for determining Mo and W content in biological samples currently involve expensive and time-consuming processes like inductively coupled plasma mass spectrometry (ICP-MS) and chelation ion chromatography. There are less intensive colorimetric methods for measuring W in abiotic samples, but these have not been adapted to biological samples like cytosolic extracts and purified proteins. Herein, we developed a colorimetric assay based on the complexation of quercetin to molybdate (MoO

Indexed as

ColorimetryColumn ChromatographyMolybdenumMolybdoenzymeQuercetinTungstenTungstoenzymeUV-Vis Spectrophotometer

Identifiers

PMID40084075
PMCPMC11896774

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.