ArticleTranslational animal science2025
Physicochemical characterization and 16S rRNA analysis of a direct-fed microbial from calf ruminal fluid and its protective effect on Sprague-Dawley rat gut barrier function.
Article in Translational animal science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Dihydromyricetin exerts neuroprotective effects in acute spinal cord injury by inhibiting NLRP3/Caspase-1 inflammasome through gut microbiome modeling.Journal of inflammation (London, England) · 2026Article
- Synthesis and Characterization of a Novel Biphenol-Based Gadolinium Complex for Encapsulation in Human Red Blood Cells.International journal of molecular sciences · 2026Article
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Authors and funding
8 authors.
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Abstract
This study aimed to characterize the physicochemical properties and microbiota composition of a direct-fed microbial (DFM) and evaluate its protective effect on intestinal permeability in Sprague-Dawley rats using fluorescein isothiocyanate dextran (FITC-d) as a biomarker. The DFM was further characterized using Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), environmental scanning electron microscopy (ESEM), energy-dispersive X-ray spectroscopy (EDS), and cell surface hydrophobicity (microbial adhesion to hexadecane [MATH] assay). The 16S rRNA gene was sequenced using short-read sequencing. In general, the DFM exhibited the characteristic FTIR bands associated with probiotic cells with a protein/carbohydrate ratio of 1.3:1. It was also found from the DLS analysis that the average particle size and surface electrical potential of the probiotic cells were 1,062 ± 77 nm and -32.6 ± 3.7 mV, respectively. ESEM studies confirmed the size of the cells (1,010 to 1,060 nm), showing a quasi-spherical cocci-type morphology, whereas EDS spectroscopy revealed a higher Nitrogen/Carbone ratio on the cell surface. Moreover, the MATH assay showed the hydrophobic character of the DFM (92% adhesion). Furthermore, based on the 16S rRNA gene analysis, the predominant genus in the DFM was
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