Evidence map›Paper›PMID 40083018›Full record

ArticleInternational journal of dermatology2025

Neutrophil Extracellular Traps Are Widely Distributed Across Lesional and Perilesional Hidradenitis Suppurativa Skin, and Elevated Serum NET Markers Associate With Moderate to Severe HS Disease.

Stephanie C M van Dalen, Josephine W J Stein, Tirza Bruurmijn, Martyn L Foster, Renato G S Chirivi, Maarten van der Linden, Helmuth van Es, Jacek C Szepietowski, Piotr K Krajewski, Kelsey R van Straalen and 3 more

Abstract read
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In one paragraph

Article in International journal of dermatology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Targeting Neutrophil Function as Therapy for Hidradenitis Suppurativa.International journal of molecular sciences · 2026
    Review
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Stephanie C M van DalenCitryll B.V., Oss, the Netherlands.ORCID https://orcid.org/0000-0003-2419-8544
Josephine W J SteinCitryll B.V., Oss, the Netherlands.
Tirza BruurmijnCitryll B.V., Oss, the Netherlands.
Martyn L FosterExperimental Pathology Consultancy, Benfleet, Essex, UK.
Renato G S ChiriviCitryll B.V., Oss, the Netherlands.ORCID https://orcid.org/0000-0003-4546-1559
Maarten van der LindenCitryll B.V., Oss, the Netherlands.
Helmuth van EsCitryll B.V., Oss, the Netherlands.
Jacek C SzepietowskiFaculty of Medicine, Wroclaw University of Science and Technology, Wrocław, Poland.ORCID https://orcid.org/0000-0003-0766-6342
Piotr K KrajewskiUniversity Centre of General Dermatology and Oncodermatology, Wrocław Medical University, Poland.ORCID https://orcid.org/0000-0003-4722-8531
Kelsey R van StraalenErasmus Medical Center, Rotterdam, the Netherlands.
Errol P PrensErasmus Medical Center, Rotterdam, the Netherlands.
John R IngramDivision of Infection & Immunity, Cardiff University, Cardiff, UK.
Eric MeldrumCitryll B.V., Oss, the Netherlands.ORCID https://orcid.org/0009-0000-8788-8038

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNeutrophils are scarce in healthy skin but infiltrate lesions of hidradenitis suppurativa (HS) patients. Activated neutrophils release proinflammatory neutrophil extracellular traps (NETs), which have been implicated in the pathophysiology of HS. This study aimed to describe the distribution of NETs relative to the features of HS skin lesions and reveal whether serum NET markers were elevated in association with disease activity.

methodsImmunohistochemistry assessed the distribution of the key NET component citrullinated histone H3 (CitH3) in lesional, perilesional, and unaffected HS skin. Several markers of NETs (nucleosomes, calprotectin, and CitH3) were quantified in HS serum with ELISA.

resultsHS lesional skin biopsies showed increased CitH3-positive staining compared to unaffected skin. This signal was widely distributed across both lesional and perilesional regions of HS skin and was associated with HS structures such as the lining of epithelialized skin tunnels. Moreover, several NET-associated markers were elevated in the serum of HS patients compared to healthy volunteers and correlated with each other. Finally, serum NET markers showed significant elevation in patients with moderate to severe disease activity based on IHS-4 scores, compared to those with no or mild activity.

conclusionsElevated NET markers are widely distributed in HS skin and serum. These data indicate that NET-associated markers in serum are candidate biomarkers for HS disease severity. The results confirm the rationale for anti-inflammatory therapy targeting NETs in HS.

Indexed as

Extracellular TrapsHidradenitis SuppurativaSkinAdultBiomarkersBiopsyCase-Control StudiesFemaleHistonesHumansImmunohistochemistryLeukocyte L1 Antigen ComplexMaleMiddle AgedNeutrophilsSeverity of Illness IndexBiomarkersHistonesLeukocyte L1 Antigen Complexhidradenitis suppurativainflammatory diseasesneutrophil extracellular trapsneutrophilic dermatosesserum NET marker

Identifiers

PMID40083018
PMCPMC12207707

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.