Evidence map›Paper›PMID 40082757›Full record

ArticleBMC genomics2025

Integrated analysis of differential expression profile of miRNA in the uterus of seasonal estrus sheep.

Fan Yang, Jiannan Liu, Yongfu La, Ran Di, Xiaoyun He, Pingqing Wang, Yufang Liu, Mingxing Chu

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Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Fan Yang *State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Rd, Beijing, 100193, China.
Jiannan Liu *School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan, 056021, China.
Yongfu LaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Rd, Beijing, 100193, China.
Ran DiState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Rd, Beijing, 100193, China.
Xiaoyun HeState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Rd, Beijing, 100193, China.
Pingqing WangCollege of Bioengineering, Chongqing University, Chongqing, 400044, China.
Yufang LiuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Rd, Beijing, 100193, China. aigaiy@126.com.
Mingxing ChuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Rd, Beijing, 100193, China. mxchu@263.net.

Funding

Agricultural Science and Technology Innovation Program of China CAAS-ZDRW202106 and ASTIP-IAS13National Natural Science Foundation of China 32172704Natural Science Foundation of Hebei Province C2019402293the China Agriculture Research System of MOF and MARA CARS-38
6 · The paper itself

Abstract

backgroundPhotoperiod is one of the important factors affecting seasonal estrus of sheep. The importance of the uterus to reproduction is self-evident. However, the uterine molecular mechanisms involved in photoperiodic regulation of seasonal estrus events in sheep remain poorly understood. In recent years, the role of uterine microRNA (miRNA) in mammalian reproduction has been continuously revealed, and it is necessary to analyze and discuss their participation in the seasonal estrus of sheep.

resultsWe compared the miRNA expression profile in uterine tissues of Sunit sheep at 3 different photoperiods, short photoperiod (SP), short transfer to long photoperiod (SLP), and long photoperiod (LP). The results showed that 31, 29 and 21 differentially expressed miRNA (DEM) were identified between SP and LP, SP and SLP, LP and SLP, respectively. Subsequently, we constructed co-expression networks of DEM and target genes in different periods. Among all DEM, nove_320, nove_338 and nove_339 target the largest number of mRNAs. Functional annotation analysis showed that these DEM target genes can be enriched in multiple GO and KEGG signaling pathways, such as cell proliferation, apoptosis, reproductive process and biological adhesion, which are related to animal reproduction and uterine receptivity. Finally, the expression level of DEM was verified by RT-qPCR, and the binding regulation relationship between DEM and their target genes in the co-expression network was confirmed by double luciferase reporter gene experiment.

conclusionsThis study revealed the changes of miRNA expression in sheep uterus under different photoperiod, providing valuable resources for understanding the molecular mechanism of sheep seasonal estrus.

Indexed as

EstrusGene Expression ProfilingMicroRNAsSeasonsTranscriptomeUterusAnimalsFemaleGene Regulatory NetworksPhotoperiodSheepMicroRNAsMicroRNAPhotoperiodSeasonal estrusSheepUterus

Identifiers

PMID40082757
PMCPMC11905465

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