Evidence map›Paper›PMID 40082743›Full record

ArticleJournal of molecular cell biology2025

Akt-elicited phosphorylation of Acapin steers cell migration.

Chunyue Wang, Jiajia Zhou, Tao Li, Shihao Du, Tetsuro Urushidani, Dongmei Wang, Yong Chen, McKay Mullen, Xinwang Cao, Shi-Yuan Cheng and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Chunyue WangMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Jiajia ZhouMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Tao LiMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Shihao DuMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.ORCID 0000-0002-1207-6542
Tetsuro UrushidaniMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Dongmei WangMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Yong ChenDepartments of Hepatobiliary Surgery and Pathology, Xijing Hospital, Xi'an 710032, China.
McKay MullenMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Xinwang CaoMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Shi-Yuan ChengThe Ken & Ruth Davee Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Xia DingDepartment of Gastroenterology, Beijing University of Chinese Medicine, Beijing 100029, China.
Fengrui YangMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Xuebiao YaoMOE Key Laboratory for Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.

Funding

Fundamental Research Funds for the Central Universities KB9100000006Fundamental Research Funds for the Central Universities KB9100000007Fundamental Research Funds for the Central Universities KB9100000013Ministry of Education IRT_17R102Ministry of Science and TechnologyNational Natural Science Foundation of China 2017YFA0503600National Natural Science Foundation of China 2022YFA1303100National Natural Science Foundation of China 31501130National Natural Science Foundation of China 31621002National Natural Science Foundation of China 32090040National Natural Science Foundation of China 32170737National Natural Science Foundation of China 32300649National Natural Science Foundation of China 32400593National Natural Science Foundation of China 91854203National Natural Science Foundation of China 91953000National Natural Science Foundation of China 92254302Plans for Major Provincial Science & Technology Projects of Anhui Province 202303a0702003
6 · The paper itself

Abstract

Cell migration requires the generation of branched actin networks and recruitment of vesicular membrane that power the protrusion of the plasma membrane in lamellipodia. However, the molecular mechanisms underlying dynamic recruitment of vesicular membrane during cell migration remain elusive. Here, we report a critical mechanism underlying epidermal growth factor (EGF)-elicited Akt signaling-steered cell migration. Using functional proteomics screen, we identified a novel ADP-ribosylation factor 6 (ARF6)-ACAP4 signaling regulator, Acapin, which inhibits the GTPase-activating protein (GAP) activity of ACAP4 to activate ARF6 GTPase in vitro. In cells, EGF stimulation elicits Akt signaling, which recruits Acapin to the lamellipodium membrane via phosphorylation of Acapin at its Ser247 residue and enhances the binding of Acapin to ACAP4 to elevate the ARF6-GTP level. Therefore, Acapin is required for efficiently stimulating cell migration by EGF-Akt signaling. Together, our results demonstrate the role of Acapin in relaying the Akt signaling cascade during cell migration processes.

Indexed as

Cell MovementProto-Oncogene Proteins c-aktADP-Ribosylation Factor 6ADP-Ribosylation FactorsAnimalsEpidermal Growth FactorGTPase-Activating ProteinsHumansMicrofilament ProteinsPhosphorylationPseudopodiaSignal TransductionVesicular Transport ProteinsADP-Ribosylation Factor 6ADP-Ribosylation FactorsARF6 protein, humanCCDC88A protein, humanEpidermal Growth FactorGTPase-Activating ProteinsMicrofilament ProteinsProto-Oncogene Proteins c-aktVesicular Transport ProteinsACAP4AcapinAktARF6 activitycell migrationEGF

Identifiers

PMID40082743
PMCPMC12570880

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.