ArticleMethods in molecular biology (Clifton, N.J.)2025
Isolation and Characterization of EVs Containing Mitochondria from OIS Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Oncogene activation triggers oncogene-induced senescence (OIS), a tumor-suppression mechanism characterized by mitochondrial dysfunction and the secretion of various factors collectively known as the senescence-associated secretory phenotype (SASP). Recent evidence highlights that extracellular vesicles (EVs), nanosized membrane-bound particles, are part of the SASP and act as a novel intercellular communication pathway. Additionally, EVs containing mitochondrial compartments are hypothesized to function as a mitochondrial quality control mechanism, eliminating damaged mitochondria in senescent cells. However, the exact role of mitochondria-enriched vesicles in OIS remains elucidated. The diversity of protocols for isolating and characterizing EVs complicates the unification of research findings. Here, we provide a concise overview of current protocols for investigating vesicles-containing mitochondria in oncogene-induced senescent cells including size exclusion chromatography, NTA analysis, flow cytometry, confocal microscopy, and Western blot.
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