Evidence map›Paper›PMID 40081372›Full record

ArticleCell systems2025

Multiplexed dynamic control of temperature to probe and observe mammalian cells.

William Benman, Pavan Iyengar, Thomas R Mumford, Zikang Huang, Manya Kapoor, Grace Liu, Lukasz J Bugaj

Abstract read
In one paragraph

Article in Cell systems, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

William BenmanDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Pavan IyengarDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.
Thomas R MumfordDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Zikang HuangDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Manya KapoorDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Grace LiuDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Lukasz J BugajDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA; Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA; Institute of Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: bugaj@seas.upenn.edu.

Funding

Harnessing protein clustering to understand, identify, and manipulate cellular systemsR35GM138211 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Lukasz Bugaj · 2020 to 2026
$3.3M
Acquisition of a Cell Sorter for Systems Biology and Cellular EngineeringS10OD026986 · OD · UNIVERSITY OF PENNSYLVANIA · PI BUGAJ, LUKASZ · 2020 to 2020
$439k
NIGMS NIH HHS R35 GM138211NIH HHS S10 OD026986
6 · The paper itself

Abstract

Temperature is an important biological stimulus, yet there is a lack of approaches to modulate the temperature of biological samples in a dynamic and high-throughput manner. The thermoPlate is a device for programmable control of temperature in a 96-well plate, compatible with cell culture and microscopy. The thermoPlate maintains feedback control of temperature independently in each well, with minutes-scale heating and cooling through ΔT = 15-20°C. We first used the thermoPlate to characterize the rapid temperature-dependent phase separation of a synthetic elastin-like polypeptide (ELP

Indexed as

TemperatureAnimalsCell Culture TechniquesElastinHeat-Shock ResponseHot TemperatureHumansElastinadaptationdeviceselastin-like polypeptidesheat shockopen sourcestress dynamicsstress granulesstress signalingtemperature modulationthermogenetics

Identifiers

PMID40081372
PMCPMC12520179

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.