Evidence map›Paper›PMID 40080297›Full record

ArticleJournal of fluorescence2025

Effect of Molecular Weight of Fluorescent Dyes on DNA Separation by Capillary Electrophoresis.

Yuqing Xu, Xin Chen, Shuaiqiang Fan, Ting Zhang, Bo Yang, Zhenqing Li, Shintaro Yamaguchi, Dawei Zhang

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuqing Xu *Engineering Research Center of Optical Instrument and System, Shanghai Key Lab of Modern Optical System, Ministry of Education, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai, 200093, China.
Xin Chen *Department of Ophthalmology and Vision Science, Eye and ENT Hospital of Fudan University, Shanghai, China.
Shuaiqiang FanEngineering Research Center of Optical Instrument and System, Shanghai Key Lab of Modern Optical System, Ministry of Education, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai, 200093, China.
Ting ZhangKey Laboratory of Myopia of State Health Ministry, and Key Laboratory of Visual Impairment and Restoration of Shanghai, Shanghai, China.
Bo YangEngineering Research Center of Optical Instrument and System, Shanghai Key Lab of Modern Optical System, Ministry of Education, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai, 200093, China.
Zhenqing LiEngineering Research Center of Optical Instrument and System, Shanghai Key Lab of Modern Optical System, Ministry of Education, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai, 200093, China. zhenqingli@163.com.
Shintaro YamaguchiGraduate School of Engineering, Department of Advanced Engineering, Chemistry and Materials Engineering Program, Nagasaki University, Nagasaki City, 852-8521, Japan.
Dawei ZhangEngineering Research Center of Optical Instrument and System, Shanghai Key Lab of Modern Optical System, Ministry of Education, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai, 200093, China.

Funding

Science and Technology Commission of Shanghai Municipality 19ZR1477500
6 · The paper itself

Abstract

Capillary electrophoresis (CE) plays an important role in the quality control of dsDNA. So far, there has been various fluorescent dyes employed for the separation of dsDNA by CE. However, the molecular weight of the dyes may affect the mass to charge ratio of dsDNA-dye complex, consequently the separation performance of dsDNA will be changed. Herein, we systematically compared the fluorescent intensity and migration times when separating the dsDNA fragments labeled or intercalated by different dyes. Results showed that the concentration of SYBR Green I affected the migration times more than Gel Green and EvaGreen, which may be caused by the lower molecular weight of EvaGreen. The optimal concentration for SYBR Green I and Gel Green is 1×, and it is 0.005× for EvaGreen. There is linear relationship between dsDNA concentration (0.1-0.5 ng/µL) and fluorescence intensity when using SYBR Green I or Gel Green for separation. Finally, we have resolved the фX174-Hinc II digest in 0.5% HEC (1300k) containing 1× SYBR within 12 min, even though there is only 6 bp difference for the adjacent dsDNA fragments. Furthermore, we also obtained the virtual dsDNA bands by OpenCV according to the electropherogram.

Indexed as

DNAFluorescent DyesBenzothiazolesDiaminesElectrophoresis, CapillaryMolecular WeightOrganic ChemicalsQuinolinesBenzothiazolesDiaminesDNAFluorescent DyesOrganic ChemicalsQuinolinesSYBR Green ICapillary ElectrophoresisDsDNAEvaGreenGel GreenNucleic AcidSYBR Green I

Identifiers

PMID40080297

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.