Evidence map›Paper›PMID 40080094›Full record

ArticleProbiotics and antimicrobial proteins2026

Development and Utilization of Quantitative Detection for Probiotics Bacillus velezensis B31 with Potential Protection Against Tomato Fusarium Wilt.

Zhenhe Su, Yanan Gao, Lemeng Dong, Yunzhuan He, Qinggang Guo, Ping Ma

Abstract read
PubMed Publisher
In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhenhe SuPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, People'S Republic of China/IPM Innovation Center of Hebei Province/International Science and Technology Joint Research Center on IPM of Hebei Province, 437# Dongguan Street, Baoding, 071000, China.
Yanan GaoPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, People'S Republic of China/IPM Innovation Center of Hebei Province/International Science and Technology Joint Research Center on IPM of Hebei Province, 437# Dongguan Street, Baoding, 071000, China.
Lemeng DongPlant Hormone Biology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, Netherlands.
Yunzhuan HeCollege of Plant Protection, Agricultural University of Hebei, Baoding, 071000, China.
Qinggang GuoPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, People'S Republic of China/IPM Innovation Center of Hebei Province/International Science and Technology Joint Research Center on IPM of Hebei Province, 437# Dongguan Street, Baoding, 071000, China. gqg77@163.com.
Ping MaPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, People'S Republic of China/IPM Innovation Center of Hebei Province/International Science and Technology Joint Research Center on IPM of Hebei Province, 437# Dongguan Street, Baoding, 071000, China.

Funding

HAAFS Science and Technology Innovation Special Project 2022KJCXZX-ZBS-1Hebei Provincial Natural Science Foundation C2021301030International Cooperation Project of Hebei Academy of Agricultural and Forestry Sciences 2023KJCXZX-ZBS-GH01National Natural Science Foundation of China 32001960National Natural Science Foundation of China 32172487S&T Program of Hebei 21326512D
6 · The paper itself

Abstract

The biocontrol efficacy of probiotics Bacillus velezensis B31 against fusarium wilt is contingent upon its successful colonization in the plant rhizosphere. This study aimed to develop a quantitative detection method for probiotics strain B31 and to monitor its rhizosphere dynamics. Utilizing comparative genomics, a specific gene in strain B31 was identified, which facilitated the design of precise primers and a Taqman probe. The resulting real-time PCR assay demonstrated high specificity to strain B31 and high sensitivity with detection limit of 28 copies/µL of the genome. Comparative analysis with regular PCR methods revealed a strong correlation, yet highlighted the superior sensitivity and rapidity of the real-time PCR technique. The temporal colonization profile of strain B31, post root-inoculation, was charted using this assay. An initial rhizosphere population of 7.2 × 10

Indexed as

BacillusFusariumPlant DiseasesProbioticsSolanum lycopersicumPlant RootsReal-Time Polymerase Chain ReactionRhizosphereBacillus velezensisBiocontrol monitoringFusarium wilt biocontrolRhizosphere colonizationStrain-specific primersUnique orf3804

Identifiers

PMID40080094

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.