Evidence map›Paper›PMID 40079179›Full record

ArticlePhysiologia plantarum

Mathematical analysis of long-distance polar auxin transport data of pin mutants questions the role of PIN1 as postulated in the chemi-osmotic theory.

Kees J M Boot, Sander C Hille, Kees R Libbenga, Marijke Libbenga-Nijkamp, Omid Karami, Bert Van Duijn, Remko Offringa

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Article in Physiologia plantarum. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Kees J M Boot *Plant Biodynamics Laboratory and Department of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Sander C Hille *Plant Biodynamics Laboratory and Department of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Kees R Libbenga *Plant Biodynamics Laboratory and Department of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Marijke Libbenga-NijkampPlant Biodynamics Laboratory and Department of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Omid KaramiDepartment of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.ORCID https://orcid.org/0000-0002-3723-4375
Bert Van DuijnPlant Biodynamics Laboratory and Department of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.ORCID https://orcid.org/0000-0003-0304-5485
Remko OffringaPlant Biodynamics Laboratory and Department of Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.ORCID https://orcid.org/0000-0002-3293-5295

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The plant hormone auxin (Indole-3-Acetic Acid, IAA) is a key player in nearly every aspect of plant growth and development ranging from cell division and cell elongation to embryogenesis and root formation. The IAA level in specific tissues and cells is regulated by synthesis, conjugation, degradation and transport. Especially long-range polar auxin transport (PAT) has been the subject of numerous studies. The chemi-osmotic theory predicts that intercellular PAT is caused by an asymmetric distribution of auxin efflux transporters in cell membranes of transporting cells, resulting in increased local membrane permeability for IAA. Members of the PIN gene family are generally considered to encode the postulated carriers. The objective of this study was to use the chemi-osmotic theory in an experimental program aimed at describing and interpreting long-range PAT data from mutants of the PIN gene family of Arabidopsis thaliana. Therefore, we put the chemi-osmotic theory in a broader theoretical framework. We find that the observed decrease in both auxin flux and transport velocity in pin1 loss-of-function mutants is not caused by decreased basal membrane permeability, as would be expected according to the chemi-osmotic theory, but is an indirect effect caused by a change in the dynamics of auxin transport due to a decrease in the expression of all four AUX1/LAX1-3 auxin influx carriers in pin1 mutants. On the basis of our findings, we conclude that the exact role of PIN1 in long-distance PAT, as postulated in the chemi-osmotic theory, should be reconsidered.

Indexed as

ArabidopsisArabidopsis ProteinsIndoleacetic AcidsMembrane Transport ProteinsBiological TransportGene Expression Regulation, PlantModels, BiologicalMutationPlant Growth RegulatorsPlant RootsArabidopsis Proteinsindoleacetic acidIndoleacetic AcidsMembrane Transport ProteinsPIN1 protein, ArabidopsisPlant Growth Regulators

Identifiers

PMID40079179
PMCPMC11904757

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