Evidence map›Paper›PMID 40078983›Full record

ArticleBiosafety and health2024

Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR.

Chudan Liang, Zequn Wang, Linjin Fan, Yulong Wang, Yuandong Zhou, Xiaofeng Yang, Jingyan Lin, Pengfei Ye, Wendi Shi, Hongxin Huang and 3 more

Abstract read
In one paragraph

Article in Biosafety and health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Evaluation of Torquetenovirus (TTV) Particle Integrity Utilizing PMAxx™.International journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Chudan LiangZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Zequn WangZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Linjin FanZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Yulong WangZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Yuandong ZhouZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Xiaofeng YangZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Jingyan LinZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Pengfei YeZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Wendi ShiZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Hongxin HuangZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Huijun YanZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Linna LiuInstitute of Infectious Disease, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510440, China.
Jun QianZhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The increase in emerging and reemerging infectious diseases has underscored the need for the prompt monitoring of intact infectious viruses and the quick assessment of their infectivity. However, molecular techniques cannot distinguish between intact infectious and noninfectious viruses. Here, two distinct methodologies have been developed for the expeditious and dependable quantification of intact infectious H1N1 virus, and several experiments have been conducted to substantiate their efficacy. One is an integrated cell absorption quantitative polymerase chain reaction (qPCR) method (ICA-qPCR), and the other is a combined propidium monoazide qPCR method (PMA-qPCR). The quantification limit is 100 cell culture infective dose 50 % (CCID

Indexed as

InactivationIntact infectious virusIntegrated cell absorption quantitative polymerase chain reaction (ICA-qPCR)Propidium monoazide quantitative polymerase chain reaction (PMA-qPCR)Rapid quantification

Identifiers

PMID40078983
PMCPMC11895024

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.