Evidence map›Paper›PMID 40078872›Full record

ArticleFrontiers in cellular and infection microbiology2025

Establishment and validation of a dual qPCR method for the detection of carbapenem-resistant

Lin Yu, Xianglan Kou, Ze Liu, Chushi Guan, Baoqing Sun

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lin YuDepartment of Clinical Laboratory, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Laboratory, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Xianglan KouKingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, Guizhou, China.
Ze LiuDepartment of Clinical Laboratory, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Laboratory, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Chushi GuanDepartment of Clinical Laboratory, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Laboratory, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Baoqing SunDepartment of Clinical Laboratory, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Laboratory, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: Bloodstream infections(BSIs) caused by carbapenem-resistant Methods: A new method was developed based on fluorescence quantitative PCR (qPCR) targeting the specific region of 16sRNA and OXA-23 gene from CRAB. The parameters were evaluated and optimized. This qPCR method was further applied in the detection of AB from 30 clinical specimens. Results: The qPCR method established in this study showed high specificity. The method successfully differentiated Conclusion: The qPCR method established in this study has strong specificity, wide linear range, good repeatability, and a lower LOD than PCR (Non-fluorescence quantification). The method provides new technical support for the early clinical diagnosis of CRAB in BSIs.

Indexed as

Acinetobacter baumanniiAcinetobacter InfectionsBacteremiaCarbapenemsReal-Time Polymerase Chain ReactionAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasesHumansLimit of DetectionMicrobial Sensitivity TestsReproducibility of ResultsRNA, Ribosomal, 16SSensitivity and SpecificityAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasesCarbapenemsRNA, Ribosomal, 16SAcinetobacter baumanniibloodstream infectionscarbapenemase resistancemethodologyqPCR

Identifiers

PMID40078872
PMCPMC11897477

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