Evidence map›Paper›PMID 40078211›Full record

ArticleFrontiers in veterinary science2025

Supplementation of DHA enhances the cryopreservation of yak semen via alleviating oxidative stress and inhibiting apoptosis.

Yanjin Zhu, Jun Yu, Xupeng Li, Zhuo Chen, Yuan Li, Yan Xiong, Honghong He, Shi Yin, Daoliang Lan, Jian Li and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yanjin ZhuKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Jun YuKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Xupeng LiKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, China.
Zhuo ChenKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, China.
Yuan LiKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Yan XiongKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Honghong HeKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Shi YinKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, China.
Daoliang LanKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, China.
Jian LiKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.
Lixue YangKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, China.
Xianrong XiongKey Laboratory for Animal Science of National Ethnic Affairs Commission, Southwest Minzu University, Chengdu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Semen cryopreservation is a crucial method for preserving genetic resources and accelerating the breeding process in domestic animals. However, the frozen-thawed process often leads to physical and chemical damage in semen, resulting in oxidative stress that diminishes sperm vitality and fertilization potential. This study aimed to explore the effects of docosahexaenoic acid (DHA) on the quality of frozen-thawed yak semen. Methods: Semen samples were collected from six healthy adult Maiwa yaks and cryopreserved in liquid nitrogen using extenders with varying DHA concentrations: 0, 0.1, 1, 10, and 100 ng/mL. After thawing, we assessed indices, antioxidant capacity, mitochondrial activity, and apoptosis status to identify the optimal DHA concentration. Results and discussion: Our findings indicate that the addition of DHA significantly improved the total motility (TM), progressive motility (PM), velocity of straight line (VSL), curvilinear velocity (VCL), and average path velocity (VAP) of cryopreserved spermatozoa, as well as the integrity of membrane and acrosome ( Conclusion: In conclusion, the incorporation of 10 ng/mL DHA into semen extenders enhances the quality and viability of yak sperm after cryopreservation by alleviating the oxidative stress, bolstering antioxidant defenses, and preserving mitochondria function, as well as inhibiting the apoptotic pathway activation.

Indexed as

antioxidantcryopreservationDHAspermyak

Identifiers

PMID40078211
PMCPMC11897752

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.