ArticleAnalytical chemistry2025
Three-Dimensional Polyglycerol-PEG-Based Hydrogels as a Universal High-Sensitivity Platform for SPR Analysis.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Metal-organic frameworks with linear and branched polyol backbones for dye removal.Scientific reports · 2026Article
- The Structure-Activity Relationship and Anticoagulation Mechanism of Polyglycerol Sulfates of Different Architectures.Biomacromolecules · 2026Article
- Sensitivity enhancement of surface plasmon resonance biosensors based on versatile nanostructures: principle, fabrication, and illustrative applications.Microsystems & nanoengineering · 2026Review
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a three-dimensional (3D) polyglycerol-poly(ethylene glycol)-based hydrogel as a new biosensing matrix for affinity analysis by surface plasmon resonance to enable a high loading of ligands for small molecule analysis while lacking a carbohydrate structure to reduce nonspecific binding. The hydrogel was synthesized by cross-linking a polyglycerol functionalized with carboxylate and maleimide groups with a dithiolated poly(ethylene glycol) by thiol-click chemistry. We demonstrated that the hydrogel coating enabled a high immobilization capacity of biomolecules and led to less nonspecific binding. Here, the degree of loading with carbonic anhydrase II and the resulting binding signal of acetazolamide were increased by a factor of 5 compared to standard CMD sensors (CM5), and the loading was comparable to CMD sensors specialized for maximum loading (CM7). This high loading capacity, combined with the reduced nonspecific binding due to the missing carbohydrate structure, presents an innovative matrix for a broad application range of surface plasmon resonance (SPR) experiments since no current commercial SPR biosensor combines these two key features.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.