Evidence map›Paper›PMID 40077521›Full record

ArticleFoods (Basel, Switzerland)2025

Immunoglobulin Y-Based Lateral Flow Immunoassay Strip Test for Detecting Ciprofloxacin Antibiotic in Raw Pork Samples.

Sumed Yadoung, Huan-Yuan Xu, Sirikwan Dokuta, Peerapong Jeeno, Pichamon Yana, Marninphan Thongkham, Korawan Sringarm, Ryoichi Ishimatsu, Zhen-Lin Xu, Surat Hongsibsong

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sumed YadoungEnvironmental Science Program, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0009-0000-5798-0132
Huan-Yuan XuGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
Sirikwan DokutaSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0003-3676-4937
Peerapong JeenoSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0002-3757-8557
Pichamon YanaEnvironmental, Occupational Health Sciences and Non-Communicable Diseases Center of Excellence, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Marninphan ThongkhamDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Korawan SringarmDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0003-0089-8183
Ryoichi IshimatsuDepartment of Applied Physics, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan.ORCID 0000-0003-2697-5167
Zhen-Lin XuGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.ORCID 0000-0002-1197-4615
Surat HongsibsongEnvironmental, Occupational Health Sciences and Non-Communicable Diseases Center of Excellence, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.ORCID 0000-0002-5510-5905

Funding

Fundamental Fund 2567 (FF67), Chiang Mai University, and Seed International Initiatives 2024, Chiang Mai University
6 · The paper itself

Abstract

Ciprofloxacin is metabolized from enrofloxacin for use in poultry to manage respiratory and gastrointestinal diseases, raising concerns due to its widespread tissue distribution and prolonged systemic persistence. This lateral flow immunoassay was designed to detect ciprofloxacin using an alternative IgY antibody binded with gold nanoparticles to detect ciprofloxacin residue in raw pork meat samples. The developed strip test achieved adequate sensitivity and specificity under the optimized conditions for pH, which is 7.8, and 20% of MeOH in 0.01 M phosphate buffer containing 1% Tween-20 was used for the buffer composition. An antibody concentration of 1.25 µg/mL was used to bind with gold nanoparticles as a probe for detection. The concentration of the test line (coating antigen) and control line (anti-IgY secondary antibody) was 0.5 mg/mL and 0.2 mg/mL, respectively. The efficiency of the developed strip test showed sensitivity with a 50% inhibitory concentration (IC

Indexed as

ciprofloxacinenrofloxacinIgY antibodyimmunoglobulin Y-basedlateral flow immunoassay strip testpolyclonal

Identifiers

PMID40077521
PMCPMC11899701

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.