A Broad-Spectrum Monoclonal Antibody-Based Heterologous ic-ELISA for the Detection of Multiple Pyrethroids in Water, Milk, Celery, and Leek.

Sulin Hou, Dandan Zhang, Zhenyu Xu, Yun Shen, Yulian Wang

Abstract read

In one paragraph

Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

A ultrasensitive immunoassay based on weakly charged AuNPs orientated antibody for triazophos detection in vegetables.Mikrochimica acta · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sulin HouNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.

Dandan ZhangNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.

Zhenyu XuNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.

Yun ShenNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.

Yulian WangNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.ORCID 0000-0003-1589-0125

Funding

Entrusted projects by enterprises and institutions 707116025

6 · The paper itself

Abstract

Pyrethroids are one of the most commonly used insecticides worldwide in agriculture, public health, and household products. To monitor the presence of pyrethroids in the environment and in food, a broad-spectrum monoclonal antibody (mAb), CL/CN-1D2, was prepared. This mAb demonstrates a 50% inhibitory concentration (IC50) for different pyrethroids: cypermethrin (129.1 µg/L), β-cypermethrin (199.6 µg/L), cyfluthrin (215.5 µg/L), fenpropathrin (220.3 µg/L), λ-cyhalothrin (226.9 µg/L), β-cyfluthrin (241.7 µg/L), deltamethrin (591.2 µg/L), and fenvalerate (763.1 µg/L). Using the mAb CL/CN-1D2, a highly sensitive heterologous indirect competitive ELISA (ic-ELISA) was developed for the rapid detection of these pyrethroids. The limit of detection (LOD) for the eight pyrethroids in water, milk, celery, and leek matrices ranged from 24.4 to 152.2 μg/kg. The recoveries ranged from 65.1% to 112.4%, with a coefficient of variation (CV) below 15%. A robust correlation (

Indexed as

celeryenzyme-linked immunosorbent assaymilkmonoclonal antibodypyrethroids

Identifiers

PMID40077471

DOI10.3390/foods14050768

PMCPMC11898949

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.