Evidence map›Paper›PMID 40076966›Full record

ArticleInternational journal of molecular sciences2025

Differentially Expressed Genes in Rat Brain Regions with Different Degrees of Ischemic Damage.

Ivan B Filippenkov, Yana Yu Shpetko, Vasily V Stavchansky, Alina E Denisova, Vadim V Yuzhakov, Natalia K Fomina, Leonid V Gubsky, Svetlana A Limborska, Lyudmila V Dergunova

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Special Issue: "New Insights of Biomarkers in Neurodegenerative Diseases".International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ivan B FilippenkovLaboratory of Human Molecular Genetics, National Research Centre "Kurchatov Institute", Kurchatov Sq. 2, Moscow 123182, Russia.ORCID 0000-0002-6964-3405
Yana Yu ShpetkoLaboratory of Human Molecular Genetics, National Research Centre "Kurchatov Institute", Kurchatov Sq. 2, Moscow 123182, Russia.ORCID 0000-0002-4586-0384
Vasily V StavchanskyLaboratory of Human Molecular Genetics, National Research Centre "Kurchatov Institute", Kurchatov Sq. 2, Moscow 123182, Russia.
Alina E DenisovaDepartment of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow 117997, Russia.
Vadim V YuzhakovA. Tsyb Medical Radiological Research Center-Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva Str. 4B, Obninsk 249036, Russia.
Natalia K FominaA. Tsyb Medical Radiological Research Center-Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva Str. 4B, Obninsk 249036, Russia.ORCID 0000-0002-1499-1349
Leonid V GubskyDepartment of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow 117997, Russia.
Svetlana A LimborskaLaboratory of Human Molecular Genetics, National Research Centre "Kurchatov Institute", Kurchatov Sq. 2, Moscow 123182, Russia.
Lyudmila V DergunovaLaboratory of Human Molecular Genetics, National Research Centre "Kurchatov Institute", Kurchatov Sq. 2, Moscow 123182, Russia.

Funding

NRC "Kurchatov Institute" The state assignmentRussian Science Foundation 24-14-00027
6 · The paper itself

Abstract

Ischemic stroke is a multifactorial disease that leads to brain tissue damage and severe neurological deficit. Transient middle cerebral artery occlusion (tMCAO) models are actively used for the molecular, genetic study of stroke. Previously, using high-throughput RNA sequencing (RNA-Seq), we revealed 3774 differentially expressed genes (DEGs) in the penumbra-associated region of the frontal cortex (FC) of rats 24 h after applying the tMCAO model. Here, we studied the gene expression pattern in the striatum that contained an ischemic focus. Striatum samples were obtained from the same rats from which we previously obtained FC samples. Therefore, we compared DEG profiles between two rat brain tissues 24 h after tMCAO. Tissues were selected based on magnetic resonance imaging (MRI) and histological examination (HE) data. As a result, 4409 DEGs were identified 24 h after tMCAO in striatum. Among them, 2609 DEGs were overlapped in the striatum and FC, whereas more than one thousand DEGs were specific for each studied tissue. Furthermore, 54 DEGs exhibited opposite changes at the mRNA level in the two brain tissues after tMCAO. Thus, the spatial regulation of the ischemic process in the ipsilateral hemisphere of rat brain at the transcriptome level was revealed. We believe that the targeted adjustment of the genome responses identified can be the key for the induction of regeneration processes in brain cells after stroke.

Indexed as

BrainBrain IschemiaGene Expression RegulationInfarction, Middle Cerebral ArteryTranscriptomeAnimalsCorpus StriatumDisease Models, AnimalGene Expression ProfilingMagnetic Resonance ImagingMaleRatsRats, Sprague-Dawleybrain damagefrontal cortexgene expressiongene networkipsilateral striatumischemic strokeRNA-seqtMCAO

Identifiers

PMID40076966
PMCPMC11900510

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.