ArticleJournal, genetic engineering & biotechnology2025
Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption.
Article in Journal, genetic engineering & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Efficient in-vitro regeneration and transformation for CRISPR/Cas9-mediated genome editing of phytoene desaturase (PDS) gene in pea (Pisum sativum L.).Plant cell reports · 2026Article
- Marker-trait associations for ideotypic traits in pigeonpea (Cajanus cajan L.).Molecular biology reports · 2026Article
- From recalcitrance to precision: a robust regeneration, transformation and targeted gene editing framework inFrontiers in genome editing · 2026Article
- The Agrobacterium-mediated genetic transformation: a gateway for efficient CRISPR/Cas9 gene editing in leguminous.Transgenic research · 2025Review
- Establishment of a TRV-based virus-induced gene silencing system using the vacuum infiltration in Tartary buckwheat.Plant cell reports · 2025Article
- Efficient CRISPR/Frontiers in plant science · 2025Article
- Development of an endogenous promoter-driven CRISPR/Cas9 system for genome editing inForestry research · 2025Article
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Authors and funding
10 authors.
Funding
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Abstract
Pigeonpea is an important legume valued for its high nutritional, agricultural, and economic significance in the Asian subcontinent. Despite its potential for high yield, productivity remains stagnant due to several abiotic and biotic stresses. To mitigate these challenges, biotechnological interventions like genome editing offer promising solutions. Towards this, developing a species-specific editing toolkit is crucial for recalcitrant species like pigeonpea. In this study, we established a CRISPR/Cas9 genome editing system targeting the phytoene desaturase (PDS) gene. We developed pigeonpea-compatible vector components, including the CcU6_7.1 promoter and an amenable Cas9 gene driven by the potato ubiquitin promoter, creating a pigeonpea-specific CRISPR/Cas9 binary vector (PP_CRISPR_pCAMBIA2301). The system was validated by Agrobacterium tumefaciens-mediated apical meristem-targeted in planta and in vitro embryonic axis explant transformations, with gene knockout confirmed by albino/bleached phenotypes. Editing efficiencies were 8.80% and 9.16% in the in planta and in vitro transformations respectively. While PCR analysis confirmed T-DNA integration, sequence analysis identified PDS gene mutations. Stability of the phenotype was demonstrated in T
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