ArticleJournal, genetic engineering & biotechnology2025
Process optimization and extraction of alkaline protease from halotolerant Streptomyces sp. VITGS3 and its use as a contact lens cleaner.
Article in Journal, genetic engineering & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Marine microbiomes and their expanding role in biotechnological potential: a systematic review.Archives of microbiology · 2026Pooled it
- Functional and structural characterization of a serine protease fromMicrobiology spectrum · 2026Article
- Enzymatic recovery of nano-silver from used X-ray films by alkaline protease fromBioengineered · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Marine halotolerant actinobacteria are robust microbes poorly explored and barely cultivable in nature. They are a trove of various secondary metabolites and enzymes, especially the alkaline proteases withstanding higher temperatures, pH, and salinity, making them an ideal source with versatile commercial and therapeutic values. This study focuses on extracting and optimizing alkaline protease production from Streptomyces sp. VITGS3 isolated from Puthuvypeen, Kerala. The protease production was optimized by Response Surface Methodology (RSM) using the Box-Behnken model, which used rice bran, wheat bran, skim milk, and casein as substrates. The maximum protease was produced (357 U/mL) using wheat bran (5.5 % w/v) as substrate at pH 9 and incubated at 45 °C for 9 days. The Michaelis-Menten model's enzyme kinetics exhibited a K
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