ArticleZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences2024
Expression and localization of HBsAg in corneas from HBsAg
Article in Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesDue to the severe shortage of donor corneas for transplantation in China, corneal component transplantation may expand the available donor pool. This study aims to evaluate the safety and feasibility of corneal component transplantation by examining the distribution of hepatitis B surface antigen (HBsAg) in corneas from HBsAg-seropositive donors under different storage media.
methodsTen corneas (from 6 donors) donated between December 2019 and March 2021 and stored at the Eye Bank of Xiangya Third Hospital, Central South University, were analyzed. Serum HBsAg levels were quantified using enzyme-linked immunosorbent assay (ELISA), while HBV DNA titers in donor blood were measured via polymerase chain reaction (PCR). Immunofluorescence staining was employed to detect HBsAg expression and its localization within corneal layers. Correlations between blood HBV DNA titers and HBsAg fluorescence intensity in corneal tissues were statistically evaluated.
resultsFour corneas from 2 HBsAg-seropositive donors and 1 cornea from 1 HBsAg-seronegative donor were preserved in medium-term preservation solution for <2 weeks. Two corneas from 1 HBsAg-seropositive donor and 2 corneas from another HBsAg-seropositive donor were preserved in glycerol for 1 month and 1 year, respectively. One HBsAg-seronegative donor cornea was stored in glycerol for 1 year. Immunofluorescence staining revealed HBsAg-positive signals in the epithelium of 2 corneas and the endothelium of 1 cornea preserved in medium-term preservation solution (<2 weeks), as well as in the epithelium of 1 cornea stored in glycerol long-term (1 year). Semi-quantitative analysis demonstrated statistically significant differences in HBsAg fluorescence intensity between the corneal epithelium and endothelium (
conclusionsHBsAg-positive expression persists in the epithelium or endothelium of donor corneas from HBsAg-seropositive individuals, regardless of preservation in medium-term preservation solution or glycerol. However, no HBsAg expression was detected in the corneal stroma. Higher blood HBV DNA titers correlate with increased likelihood of HBsAg positivity in corneal tissues. This study lays the groundwork for further investigation into the potential use of HBsAg-positive donor corneas in lamellar keratoplasty.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.