Evidence map›Paper›PMID 40073995›Full record

ArticleEuropean journal of dentistry2025

In vitro Evaluation of Cytotoxicity, Biocompatibility, and Osteogenic Effect of Two Calcium Silicate Sealers Compared with AH Plus Jet.

Monique Aparecida de Lima Rios Pitzschk, Carlos Eduardo da Silveira Bueno, Carlos Eduardo Fontana, Alexandre Sigrist De Martin, Daniel Guimarães Pedro Rocha, Carolina Pessoa Stringheta, Ana Grasiela da Silva Limoeiro, Virgilio Marcio Bastos Braga, Caroliny Chavier Guimaraes, Marilia Fagury Videira Marceliano-Alves and 3 more

Abstract read
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In one paragraph

Article in European journal of dentistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Monique Aparecida de Lima Rios PitzschkFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Carlos Eduardo da Silveira BuenoFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Carlos Eduardo FontanaPUC-Campinas, Center of Life Sciences, Postgraduate Program in Health Sciences, Campinas, São Paulo, Brazil.
Alexandre Sigrist De MartinFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Daniel Guimarães Pedro RochaPontifical Catholic University of Campinas, Department of Endodontics, School of Life Sciences, Campinas, São Paulo, Brazil.
Carolina Pessoa StringhetaFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Ana Grasiela da Silva LimoeiroDepartment of Restorative Dentistry, Dental Materials, and Endodontics, Bauru School of Dentistry, University of São, Bauru, Brazil.ORCID 0000-0003-4633-720X
Virgilio Marcio Bastos BragaFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Caroliny Chavier GuimaraesPostgraduate Program in Dentistry, Iguaçu University, Nova Iguaçu, RJ, Brazil.
Marilia Fagury Videira Marceliano-AlvesPostgraduate Program in Dentistry, Iguaçu University, Nova Iguaçu, RJ, Brazil.ORCID 0000-0002-2917-5934
Wayne Martins NascimentoFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Thiago Sena GuimarãesMauricio de Nassau University Center, Dentistry Department, Rio de Janeiro, Brazil.ORCID 0000-0003-3500-6755
Rina Andrea PelegrineFaculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.ORCID 0000-0003-4175-2121

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe purpose of the study was to investigate the cytotoxicity, biocompatibility, and osteogenic effect of EndoSequence BCSealer HiFlow (BCH) and Bio-C Sealer (BCS) in osteosarcoma cells SAOS-2 compared with AH Plus Jet. MATERIALS AND

methodsFor cytotoxicity analysis, the [3-(4,5-dimethyl-thiazole)-2,5-diphenyltetrazolium bromide-MTT; Sigma/Aldrich] method was used after 24, 48, and 72 hours. For cellular bioactivity, alkaline phosphatase enzyme (ALP) was evaluated after 7 and 14 days. STATISTICAL ANALYSIS: MTT assay was assessed using analysis of variance (ANOVA) and Tukey's test and over time using ANOVA and Sidak's test. For ALP analysis, the one-way ANOVA and Student's paired t-tests were used.

resultsBCS was like AH Plus Jet. After 72 hours, BCH and BCS did not differ from AH Plus Jet and showed similar behavior in terms of viability. ALP showed a difference only after 14 days. BCH had the lowest bioactivity value. AH Plus Jet and BCS were like each other.

conclusionAHP was more viable for SAOS-2, and the biocompatibility of Calcium silicate-based sealers was acceptable. The present finding assessed the biocompatibility of Calcium silicate bioceramic sealers, such as BCS, EndoSequence BCH, and AH Plus Jet, is acceptable and they have osteogenic effect and bioactivity.

Identifiers

PMID40073995
PMCPMC12182430

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.