Evidence map›Paper›PMID 40073020›Full record

ArticleSTAR protocols2025

Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy colostrum and milk proteome.

Shashank M Patil, Ramith Ramu, Khang Wen Goh

Abstract read
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shashank M PatilDepartment of Biotechnology & Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India.
Ramith RamuDepartment of Biotechnology & Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India. Electronic address: ramithramu@jssuni.edu.in.
Khang Wen GohFaculty of Data Science and Information Technology, INTI International University, Nilai 71800, Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colostrum and milk from dairy sources consist of whey, casein, and fat, which have notable pharmacological properties due to their proteins and peptides. Here, we present a protocol for isolating, simulating in vitro gastrointestinal digestion, and fractionating colostrum and milk hydrolysates from any dairy source. We also describe steps for nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC-ESI-MS/MS) identification of proteins and peptides and in silico system biology-based profiling of the proteins and peptides present in the hydrolysates.

Indexed as

ColostrumGastrointestinal TractMilkMilk ProteinsProteomeProteomicsSystems BiologyAnimalsChromatography, LiquidSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryMilk ProteinsProteomeMass SpectrometryProtein BiochemistrySystems biology

Identifiers

PMID40073020
PMCPMC11946872

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.