Evidence map›Paper›PMID 40073019›Full record

ArticleSTAR protocols2025

Protocol for simultaneous detection of PRRS virus infection and dying cell populations by a flow cytometry double-staining approach.

Michelle Steyn, Steven R Lawson, Eric Nelson, Gergely Imre

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Michelle SteynDepartment of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USA.
Steven R LawsonDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57006, USA.
Eric NelsonDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57006, USA.
Gergely ImreDepartment of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USA. Electronic address: gergely.imre@sdstate.edu.

Funding

Transcriptome & Networks Analysis CoreP20GM135008 · NIGMS · SOUTH DAKOTA STATE UNIVERSITY · PI Adam David Hoppe · 2022 to 2026
$13.5M
NIGMS NIH HHS P20 GM135008
6 · The paper itself

Abstract

When investigating cellular responses to viral infections, there is a need to quantitatively distinguish between dying cells, infected cells, and cells that are both infected and dying. Here, we present a protocol to simultaneously detect porcine reproductive and respiratory syndrome virus (PRRSV) infection and cell death using a flow cytometry double-staining approach. We describe steps for cell seeding, infection, and collection; staining dead cells; sample fixation; and cell permeabilization. We then detail procedures for immunostaining and flow cytometry analysis.

Indexed as

Flow CytometryPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusStaining and LabelingAnimalsCell DeathSwineCell BiologyCell cultureFlow CytometryMicrobiologyModel Organisms

Identifiers

PMID40073019
PMCPMC11950744

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.