Evidence map›Paper›PMID 40072727›Full record

ArticleFish physiology and biochemistry2025

Development of colloidal gold test strip based on the BsVg819 gene fragment of vitellogenin of Bostrichthys sinensis for the detection of vitellogenin in multiple fish species.

Jiahang Mu, Tingting Zhou, Kun Meng, Yangyang Zhang, Junhua Fang, Kefu Zhou

Abstract read
PubMed Publisher
In one paragraph

Article in Fish physiology and biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jiahang Mu *Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.
Tingting Zhou *Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.
Kun MengKey Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.
Yangyang ZhangKey Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.
Junhua FangKey Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.
Kefu ZhouKey Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China. zhkefu@xmu.edu.cn.

Funding

National Key Research and Development Program of China 2017YFCO506100Xiamen Boson Biotech Co., Ltd XDHT2020603A
6 · The paper itself

Abstract

As an environmental estrogen biomarker, the yolk precursor, vitellogenin (Vtg) is widely used in the assessment of estrogen pollution in aquatic environment. Currently, the detection of Vtg in plasma is mainly achieved by enzyme-linked immunosorbent assay (ELISA) method based on Vtg antibodies. However, due to differences in the immunological epitopes of Vtg from various species, Vtg antibodies have low universality. Therefore, identifying a universal antigenic epitopes of Vtg from multiple fish species and designing a tools that can be applied in the field can promote the use of Vtg in monitoring estrogenic contamination in aquatic environments. Bioinformatics analysis of the Vtg of Bostrichthys sinensis revealed that the protein is highly conserved in structure. The results of PCR showed that the amino acid sequence encoded by the BsVg819 gene fragment from the Vtg gene of Bostrichthys sinensis could have more than 97% similarity with the amino acid sequences of the PCR products of ten fish species. Development of a colloidal gold immunochromatographic test strip using recombinant proteins was expressed in BsVg819 gene fragments. The test strip was able to detect Vtg in the plasma of untreated female Bostrichthys sinensis and ten different female fish species. Vtg in the plasma of juvenile Bostrichthys sinensis treated with estrogen is elevated and can be detected by test strips. The results show that the test strips have good usability. Compared to ELISA, the strip is prospective for field applications. It provides a portable tool for future rapid detection of estrogenic contamination in the field.

Indexed as

FishesGold ColloidPerciformesVitellogeninsAmino Acid SequenceAnimalsEnzyme-Linked Immunosorbent AssayFemaleReagent StripsGold ColloidReagent StripsVitellogenins17β-estradiolBiomarkersBostrichthys sinensisEnvironmental estrogenUniversal epitopeVitellogenin

Identifiers

PMID40072727

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.